TS1/22.1.1.13.3

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SKU: TS1/22.1.1.13.3
View product citations for antibody TS1/22.1.1.13.3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD11a (Integrin alpha-L, LFA-1 alpha subunit), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Springer, T.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Center for Blood Research
Positive Tested Species Reactivity: Human
Depositors Notes: Blocks CTL killing, ADCC killing of NK & granulocytes, homotypic B cell adhesion, gammaINF adhesion of monocytes, leukocyte-epithelial cell interactions. Doesn't appear to work for western blotting. Binds to all leukocytes.
Antigen Molecular Weight: Predicted: 129 kDa; Apparent: 180 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ITGAL
Immunogen: Human HLA-DRCTL line cytolytic T-lymphocytes.
Alternate Gene Names: LFA-1
Alternate Antibody Name: TS1/22
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Location or Sequence: I domain (aa 248-303)
Uniprot ID: P20701 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3683 
Additional Characterization:  
Antibody Registry ID: AB_528132 
Additional Information:
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TS1/22.1.1.13.3 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product TS1/22.1.1.13.3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

17 References

  • Initial Publication
  • IF References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Springer TA
    Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.

    IF References

    Role of the LFA-1 molecule in cellular interactions required for antibody production in humans.
    Griscelli C
    Journal of immunology (Baltimore, Md. : 1950) 136.9 (1986 May 1): 3198-203.

    CD18 activation epitopes induced by leukocyte activation.
    Staunton DE
    Journal of immunology (Baltimore, Md. : 1950) 167.11 (2001 Dec 1): 6113-22.

    IP References
    FB References

    Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Springer TA
    Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.

    T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1.
    Springer TA
    Nature 341.6243 (1989 Oct 19): 619-24.

    Characterization of the adherence of human monocytes to cytokine-stimulated human macrovascular endothelial cells.
    Van Furth R
    Immunology 74.4 (1991 Dec): 661-9.

    Characterization of pig intercellular adhesion molecule-2 and its interaction with human LFA-1.
    Cowan PJ
    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 4.4 (2004 Apr): 515-25.

    Role of the LFA-1 molecule in cellular interactions required for antibody production in humans.
    Griscelli C
    Journal of immunology (Baltimore, Md. : 1950) 136.9 (1986 May 1): 3198-203.

    Antibodies against the CD44 p80, lymphocyte homing receptor molecule augment human peripheral blood T cell activation.
    Haynes BF
    Journal of immunology (Baltimore, Md. : 1950) 144.1 (1990 Jan 1): 7-15.

    Suppression of human lymphocyte chemotaxis and transendothelial migration by anti-LFA-1 antibody.
    Anderson DC
    Journal of immunology (Baltimore, Md. : 1950) 143.10 (1989 Nov 15): 3207-10.

    TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen.
    Levy R
    Journal of immunology (Baltimore, Md. : 1950) 145.7 (1990 Oct 1): 2207-13.

    CD43 interferes with T-lymphocyte adhesion.
    Staunton DE
    Proceedings of the National Academy of Sciences of the United States of America 89.11 (1992 Jun 1): 5001-5.

    Activation of lymphocyte function-associated molecule-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) mimicked by an antibody directed against CD18.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 155.2 (1995 Jul 15): 854-66.

    CD18 activation epitopes induced by leukocyte activation.
    Staunton DE
    Journal of immunology (Baltimore, Md. : 1950) 167.11 (2001 Dec 1): 6113-22.

    FACS References

    Suppression of human lymphocyte chemotaxis and transendothelial migration by anti-LFA-1 antibody.
    Anderson DC
    Journal of immunology (Baltimore, Md. : 1950) 143.10 (1989 Nov 15): 3207-10.

    Epitope mapping of antibodies to the C-terminal region of the integrin beta 2 subunit reveals regions that become exposed upon receptor activation.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 166.9 (2001 May 1): 5629-37.

    T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1.
    Springer TA
    Nature 341.6243 (1989 Oct 19): 619-24.

    Activation of lymphocyte function-associated molecule-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) mimicked by an antibody directed against CD18.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 155.2 (1995 Jul 15): 854-66.

    A human CD4- T cell leukemia subclone with contact-dependent helper function.
    Lederman S
    Journal of immunology (Baltimore, Md. : 1950) 147.10 (1991 Nov 15): 3389-95.

    Epitope Map References
    All References

    Role of the LFA-1 molecule in cellular interactions required for antibody production in humans.
    Griscelli C
    Journal of immunology (Baltimore, Md. : 1950) 136.9 (1986 May 1): 3198-203.

    CD18 activation epitopes induced by leukocyte activation.
    Staunton DE
    Journal of immunology (Baltimore, Md. : 1950) 167.11 (2001 Dec 1): 6113-22.

    Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Springer TA
    Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.

    Activation of lymphocyte function-associated molecule-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) mimicked by an antibody directed against CD18.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 155.2 (1995 Jul 15): 854-66.

    A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.
    Springer TA
    The Journal of experimental medicine 158.6 (1983 Dec 1): 1785-1803.

    T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1.
    Springer TA
    Nature 341.6243 (1989 Oct 19): 619-24.

    Characterization of the adherence of human monocytes to cytokine-stimulated human macrovascular endothelial cells.
    Van Furth R
    Immunology 74.4 (1991 Dec): 661-9.

    Characterization of pig intercellular adhesion molecule-2 and its interaction with human LFA-1.
    Cowan PJ
    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 4.4 (2004 Apr): 515-25.

    Antibodies against the CD44 p80, lymphocyte homing receptor molecule augment human peripheral blood T cell activation.
    Haynes BF
    Journal of immunology (Baltimore, Md. : 1950) 144.1 (1990 Jan 1): 7-15.

    Suppression of human lymphocyte chemotaxis and transendothelial migration by anti-LFA-1 antibody.
    Anderson DC
    Journal of immunology (Baltimore, Md. : 1950) 143.10 (1989 Nov 15): 3207-10.

    TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen.
    Levy R
    Journal of immunology (Baltimore, Md. : 1950) 145.7 (1990 Oct 1): 2207-13.

    CD43 interferes with T-lymphocyte adhesion.
    Staunton DE
    Proceedings of the National Academy of Sciences of the United States of America 89.11 (1992 Jun 1): 5001-5.

    Epitope mapping of antibodies to the C-terminal region of the integrin beta 2 subunit reveals regions that become exposed upon receptor activation.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 166.9 (2001 May 1): 5629-37.

    A human CD4- T cell leukemia subclone with contact-dependent helper function.
    Lederman S
    Journal of immunology (Baltimore, Md. : 1950) 147.10 (1991 Nov 15): 3389-95.

    A binding interface on the I domain of lymphocyte function-associated antigen-1 (LFA-1) required for specific interaction with intercellular adhesion molecule 1 (ICAM-1).
    Springer TA
    The Journal of biological chemistry 270.32 (1995 Aug 11): 19008-16.

    Spatiotemporal segregation of endothelial cell integrin and nonintegrin extracellular matrix-binding proteins during adhesion events.
    Madri JA
    The Journal of cell biology 110.3 (1990 Mar): 789-801.

    The B cell antigen receptor controls integrin activity through Btk and PLCgamma2.
    Pals ST
    The Journal of experimental medicine 198.10 (2003 Nov 17): 1539-50.

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