TS2/18.1.1

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SKU: TS2/18.1.1

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: TS2/18.1.1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: CD2
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2244335 
Uniprot ID: P06729 
RRID:  
Entrez Gene ID: 914 
Clonality:
Immunogen: T-lymphocytes, cytolytic (CTL)
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS1
Epitope Mapped: Yes
Antigen Name: T-cell surface antigen CD2 (LFA-2)
Epitope Location or Sequence: a.a. 87-99
Alternate Antigen Name:
Deposit Date: 4/4/1989
Antigen Molecular Weight: 49 kDa/ 36 kDa
Depositor: Springer, T.A.
Antigen Sequence:
Depositor Institution: Harvard University
Antigen Species: Human
Depositor Notes: This antibody does not recognize mouse CD2.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Rat, Sheep
Additional Information: This antibody has been used as a tag marker for transposon UAS-CD2-HRP/CyO in Drosophila (PMID: 17029252). RRID:AB_2244335
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Function Blocking, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TS2/18.1.1 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product TS2/18.1.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Springer TA
    Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.

    IF References

    Quantitative changes in integrin and focal adhesion signaling regulate myoblast cell cycle withdrawal.
    Horwitz AF
    The Journal of cell biology 144.6 (1999 Mar 22): 1295-309.

    Identification of the TS2/18-recognized epitope on the CD2 molecule as a target for suppression of T cell cytokine synthesis.
    Holter W
    Journal of immunology (Baltimore, Md. : 1950) 154.11 (1995 Jun 1): 5813-20.

    WB References

    Quantitative changes in integrin and focal adhesion signaling regulate myoblast cell cycle withdrawal.
    Horwitz AF
    The Journal of cell biology 144.6 (1999 Mar 22): 1295-309.

    Identification of the TS2/18-recognized epitope on the CD2 molecule as a target for suppression of T cell cytokine synthesis.
    Holter W
    Journal of immunology (Baltimore, Md. : 1950) 154.11 (1995 Jun 1): 5813-20.

    IHC References

    Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Springer TA
    Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.

    IP References

    Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Springer TA
    Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.

    Quantitative changes in integrin and focal adhesion signaling regulate myoblast cell cycle withdrawal.
    Horwitz AF
    The Journal of cell biology 144.6 (1999 Mar 22): 1295-309.

    FB References

    Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Springer TA
    Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.

    Specific interaction of lymphocyte function-associated antigen 3 with CD2 can inhibit T cell responses.
    Wallner BP
    The Journal of experimental medicine 178.1 (1993 Jul 1): 211-22.

    Identification of the TS2/18-recognized epitope on the CD2 molecule as a target for suppression of T cell cytokine synthesis.
    Holter W
    Journal of immunology (Baltimore, Md. : 1950) 154.11 (1995 Jun 1): 5813-20.

    CD1-restricted microbial lipid antigen-specific recognition found in the CD8+ alpha beta T cell pool.
    Brenner MB
    Journal of immunology (Baltimore, Md. : 1950) 162.1 (1999 Jan 1): 366-71.

    Human tonsillar dendritic cell-induced T cell responses: analysis of molecular mechanisms using monoclonal antibodies.
    Katz DR
    European journal of immunology 19.4 (1989 Apr): 581-7.

    CD8 alpha beta T cells are not essential to the pathogenesis of arthritis or colitis in HLA-B27 transgenic rats.
    Taurog JD
    Journal of immunology (Baltimore, Md. : 1950) 170.2 (2003 Jan 15): 1099-105.

    FACS References

    Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Springer TA
    Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.

    Quantitative changes in integrin and focal adhesion signaling regulate myoblast cell cycle withdrawal.
    Horwitz AF
    The Journal of cell biology 144.6 (1999 Mar 22): 1295-309.

    Identification of the TS2/18-recognized epitope on the CD2 molecule as a target for suppression of T cell cytokine synthesis.
    Holter W
    Journal of immunology (Baltimore, Md. : 1950) 154.11 (1995 Jun 1): 5813-20.

    Epitope Map References

    Specific interaction of lymphocyte function-associated antigen 3 with CD2 can inhibit T cell responses.
    Wallner BP
    The Journal of experimental medicine 178.1 (1993 Jul 1): 211-22.

    All References

    Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Springer TA
    Proceedings of the National Academy of Sciences of the United States of America 79.23 (1982 Dec): 7489-93.

    Quantitative changes in integrin and focal adhesion signaling regulate myoblast cell cycle withdrawal.
    Horwitz AF
    The Journal of cell biology 144.6 (1999 Mar 22): 1295-309.

    Identification of the TS2/18-recognized epitope on the CD2 molecule as a target for suppression of T cell cytokine synthesis.
    Holter W
    Journal of immunology (Baltimore, Md. : 1950) 154.11 (1995 Jun 1): 5813-20.

    Specific interaction of lymphocyte function-associated antigen 3 with CD2 can inhibit T cell responses.
    Wallner BP
    The Journal of experimental medicine 178.1 (1993 Jul 1): 211-22.

    CD1-restricted microbial lipid antigen-specific recognition found in the CD8+ alpha beta T cell pool.
    Brenner MB
    Journal of immunology (Baltimore, Md. : 1950) 162.1 (1999 Jan 1): 366-71.

    Human tonsillar dendritic cell-induced T cell responses: analysis of molecular mechanisms using monoclonal antibodies.
    Katz DR
    European journal of immunology 19.4 (1989 Apr): 581-7.

    CD8 alpha beta T cells are not essential to the pathogenesis of arthritis or colitis in HLA-B27 transgenic rats.
    Taurog JD
    Journal of immunology (Baltimore, Md. : 1950) 170.2 (2003 Jan 15): 1099-105.

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