Hybridoma Cells Available: No
Antigen Species: Drosophila
Depositor: Bejsovec, A.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: Duke University - Durham, NC
Positive Tested Species Reactivity: Drosophila
Predicted Species Reactivity:
Immunogen: Recombinant 6XHis-tumbleweed fusion protein
Alternate Gene Names: Dmel_CG13345
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP 2/0 Ag-14
Epitope Location or Sequence:
Uniprot ID: A1Z9I5
Immunogen Sequence: Full length protein
Entrez Gene ID: 36538
Antibody Registry ID: AB_2617200
Additional Information: RRID:AB_2617200 FBgn0086356.
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Tum 1H5 was deposited to the DSHB by Bejsovec, A. (DSHB Hybridoma Product Tum 1H5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.