Product Name/ID: Tum 1H5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: tum
Ab Isotype: MIgG1, kappa light chain
Entrez Gene ID: 36538
Immunogen: Recombinant 6XHis-tumbleweed fusion protein
Immunogen Sequence: Full length protein
Myeloma Strain: SP 2/0 Ag-14
Epitope Mapped: No
Antigen Name: Tumbleweed/FI24033p1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 10/7/2011
Antigen Molecular Weight: 69.7 kDa
Depositor: Bejsovec, A.
Depositor Institution: Duke University
Antigen Species: Drosophila
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Drosophila
Additional Information: RRID:AB_2617200 FBgn0086356.
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Tum 1H5 was deposited to the DSHB by Bejsovec, A. (DSHB Hybridoma Product Tum 1H5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).