UNC60-26.2.1

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$40.00
SKU: UNC60-26.2.1-s
View product citations for antibody UNC60-26.2.1 on CiteAb

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Available: 38

DSHB Data Sheet

Catalog Fields

Antigen: Regulator of G-protein signaling 12 (RGS12)
Hybridoma Cells Available: No
Antigen Species: Rat
Depositor: Siderovski, D.P.
Isotype: MIgG2b, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: UNC-Chapel Hill
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes:
Antigen Molecular Weight: 160 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Rgs12
Immunogen: N-terminal peptide PDZ & PTB domains
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63.AG8.653
Epitope Location or Sequence:
Uniprot ID: O08774 
Immunogen Sequence: Protein domain
Entrez Gene ID: 54292 
Additional Characterization:  
Antibody Registry ID: AB_2619578 
Additional Information: RRID:AB_2619577
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
UNC60-26.2.1 was deposited to the DSHB by Siderovski, D.P. (DSHB Hybridoma Product UNC60-26.2.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Differential expression of regulator of G-protein signaling R12 subfamily members during mouse development.
    D'Souza SJ
    Developmental dynamics : an official publication of the American Association of Anatomists 234.2 (2005 Oct): 438-44.

    IF References

    Differential expression of regulator of G-protein signaling R12 subfamily members during mouse development.
    D'Souza SJ
    Developmental dynamics : an official publication of the American Association of Anatomists 234.2 (2005 Oct): 438-44.

    WB References

    Differential expression of regulator of G-protein signaling R12 subfamily members during mouse development.
    D'Souza SJ
    Developmental dynamics : an official publication of the American Association of Anatomists 234.2 (2005 Oct): 438-44.

    Selective role for RGS12 as a Ras/Raf/MEK scaffold in nerve growth factor-mediated differentiation.
    Siderovski DP
    The EMBO journal 26.8 (2007 Apr 18): 2029-40.

    All References

    Differential expression of regulator of G-protein signaling R12 subfamily members during mouse development.
    D'Souza SJ
    Developmental dynamics : an official publication of the American Association of Anatomists 234.2 (2005 Oct): 438-44.

    Selective role for RGS12 as a Ras/Raf/MEK scaffold in nerve growth factor-mediated differentiation.
    Siderovski DP
    The EMBO journal 26.8 (2007 Apr 18): 2029-40.

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