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SKU: VI-E6-2C6-s
View product citations for antibody VI-E6-2C6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Protein phosphatase 2, regulatory subunit B, beta
Hybridoma Cells Available: No
Antigen Species: Rat
Depositor: Strack, Stefan
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Mammal
Depositors Notes: This antibody specifically recognizes ectopically expressed B 2, but not B 1.
Antigen Molecular Weight: 51.7 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: PPP2R2B
Immunogen: N-terminal synthetic peptide conjugated to KLH
Alternate Gene Names: PR52B, PR55-BETA, SCA12
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: a.a. 2-21 Bbeta2 (isoform 2)
Uniprot ID: Q00005 
Immunogen Sequence: KCFSRYLPYIFRPPNTILSS; a.a. 2-21 of isoform 2
Entrez Gene ID: 5521 
Additional Characterization:  
Antibody Registry ID: AB_2722486 
Additional Information: RRID:AB_2722486
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
VI-E6-2C6 was deposited to the DSHB by Strack, Stefan (DSHB Hybridoma Product VI-E6-2C6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
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