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SKU: VI-E6-2C6-s
View product citations for antibody VI-E6-2C6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Protein phosphatase 2, regulatory subunit B, beta
Hybridoma Cells Available: No
Antigen Species: Rat
Depositor: Strack, Stefan
Isotype: MIgG2a
Depositors Institution: University of Iowa
Host Species: mouse
Depositors Notes: This antibody specifically recognizes ectopically expressed B 2, but not B 1.
Positive Tested Species Reactivity: Mammal
Gene: PPP2R2B
Antigen Molecular Weight: 51.7 kDa
Alternate Gene Names: PR52B, PR55-BETA, SCA12
Immunogen: N-terminal synthetic peptide conjugated to KLH
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: Q00005 
Epitope Location or Sequence: a.a. 2-21 Bbeta2 (isoform 2)
Entrez Gene ID: 5521 
Immunogen Sequence: KCFSRYLPYIFRPPNTILSS; a.a. 2-21 of isoform 2
Antibody Registry ID: AB_2722486 
Additional Information: RRID:AB_2722486
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
VI-E6-2C6 was deposited to the DSHB by Strack, Stefan (DSHB Hybridoma Product VI-E6-2C6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

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  • IF References
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