Wind 4G7

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$40.00
SKU: Wind 4G7
View product citations for antibody Wind 4G7 on CiteAb

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Available: 18

DSHB Data Sheet

Catalog Fields

Antigen: Windbeutel protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Schupbach, T.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University - Princeton, NJ
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 29.4 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: wbl
Immunogen: Recombinant GST-fusion protein domain
Alternate Gene Names: Dmel_CG7225
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: O44342 
Immunogen Sequence: a.a. 22-257
Entrez Gene ID: 37206 
Additional Characterization:
Antibody Registry ID: AB_10584800 
Additional Information: RRID:AB_10584800 FBgn0004003.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Wind 4G7 was deposited to the DSHB by Schupbach, T. (DSHB Hybridoma Product Wind 4G7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    All References

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