XAN-3 (Clone 6F11)

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SKU: XAN-3 (Clone 6F11)-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: XAN-3 (Clone 6F11)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: ncam1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID:  
Uniprot ID: P16170 
RRID:  
Entrez Gene ID: 100038291 
Clonality: Monoclonal
Immunogen: Stage 45-53 optic nerves
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: NCAM, extracellular domain; NCAM-180
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/27/1995
Antigen Molecular Weight: 180-200 kDa
Depositor: Sakaguchi, Donald S.
Antigen Sequence:
Depositor Institution: Iowa State University
Antigen Species: Xenopus
Depositor Notes: This antibody labels embryonic and mature retinal neuron somata, dendrites and axons, but does label photoreceptor outer segments. The antibody does not cross-react with chick.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Xenopus
Additional Information: XAN-3 6F11 works well for IHC/IF in paraformaldehyde fixed tissue.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
XAN-3 (Clone 6F11) was deposited to the DSHB by Sakaguchi, Donald S. (DSHB Hybridoma Product XAN-3 (Clone 6F11))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

6 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Growth cone interactions with a glial cell line from embryonic Xenopus retina.
    Harris WA
    Developmental biology 134.1 (1989 Jul): 158-74.

    IF References
    IHC References

    Basic fibroblast growth factor (FGF-2) induced transdifferentiation of retinal pigment epithelium: generation of retinal neurons and glia.
    Reh TA
    Developmental dynamics : an official publication of the American Association of Anatomists 209.4 (1997 Aug): 387-98.

    The expression of XIF3 in undifferentiated anterior neuroectoderm, but not in primary neurons, is induced by the neuralizing agent noggin.
    Sharpe CR
    The International journal of developmental biology 42.6 (1998 Sep): 757-62.

    A role for S1P signalling in axon guidance in the Xenopus visual system.
    Holt CE
    Development (Cambridge, England) 135.2 (2008 Jan): 333-42.

    XBF-2 is a transcriptional repressor that converts ectoderm into neural tissue.
    Harland RM
    Development (Cambridge, England) 125.24 (1998 Dec): 5019-31.

    All References

    Basic fibroblast growth factor (FGF-2) induced transdifferentiation of retinal pigment epithelium: generation of retinal neurons and glia.
    Reh TA
    Developmental dynamics : an official publication of the American Association of Anatomists 209.4 (1997 Aug): 387-98.

    The expression of XIF3 in undifferentiated anterior neuroectoderm, but not in primary neurons, is induced by the neuralizing agent noggin.
    Sharpe CR
    The International journal of developmental biology 42.6 (1998 Sep): 757-62.

    A role for S1P signalling in axon guidance in the Xenopus visual system.
    Holt CE
    Development (Cambridge, England) 135.2 (2008 Jan): 333-42.

    XBF-2 is a transcriptional repressor that converts ectoderm into neural tissue.
    Harland RM
    Development (Cambridge, England) 125.24 (1998 Dec): 5019-31.

    Math5 encodes a murine basic helix-loop-helix transcription factor expressed during early stages of retinal neurogenesis.
    Glaser T
    Development (Cambridge, England) 125.23 (1998 Dec): 4821-33.

    Growth cone interactions with a glial cell line from embryonic Xenopus retina.
    Harris WA
    Developmental biology 134.1 (1989 Jul): 158-74.

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