XAN-3 (Clone 6F11)

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$45.00
SKU: XAN-3 (Clone 6F11)-s
View product citations for antibody XAN-3 (Clone 6F11) on CiteAb

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Available: 34

DSHB Data Sheet

Catalog Fields

Antigen: NCAM, extracellular domain; NCAM-180
Hybridoma Cells Available: No
Antigen Species: Xenopus
Depositor: Sakaguchi, Donald S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Iowa State University
Positive Tested Species Reactivity: Xenopus
Depositors Notes: This antibody labels embryonic and mature retinal neuron somata, dendrites and axons, but does label photoreceptor outer segments. The antibody does not cross-react with chick.
Antigen Molecular Weight: 180-200 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ncam1
Immunogen: Stage 45-53 optic nerves
Alternate Gene Names: ncam1-b , ncam1-a
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P16170 
Immunogen Sequence:
Entrez Gene ID: 100038291 
Additional Characterization:  
Antibody Registry ID:  
Additional Information: XAN-3 6F11 works well for IHC/IF in paraformaldehyde fixed tissue.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
XAN-3 (Clone 6F11) was deposited to the DSHB by Sakaguchi, Donald S. (DSHB Hybridoma Product XAN-3 (Clone 6F11))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Growth cone interactions with a glial cell line from embryonic Xenopus retina.
    Harris WA
    Developmental biology 134.1 (1989 Jul): 158-74.

    IF References
    IHC References

    Basic fibroblast growth factor (FGF-2) induced transdifferentiation of retinal pigment epithelium: generation of retinal neurons and glia.
    Reh TA
    Developmental dynamics : an official publication of the American Association of Anatomists 209.4 (1997 Aug): 387-98.

    The expression of XIF3 in undifferentiated anterior neuroectoderm, but not in primary neurons, is induced by the neuralizing agent noggin.
    Sharpe CR
    The International journal of developmental biology 42.6 (1998 Sep): 757-62.

    A role for S1P signalling in axon guidance in the Xenopus visual system.
    Holt CE
    Development (Cambridge, England) 135.2 (2008 Jan): 333-42.

    XBF-2 is a transcriptional repressor that converts ectoderm into neural tissue.
    Harland RM
    Development (Cambridge, England) 125.24 (1998 Dec): 5019-31.

    All References

    Basic fibroblast growth factor (FGF-2) induced transdifferentiation of retinal pigment epithelium: generation of retinal neurons and glia.
    Reh TA
    Developmental dynamics : an official publication of the American Association of Anatomists 209.4 (1997 Aug): 387-98.

    The expression of XIF3 in undifferentiated anterior neuroectoderm, but not in primary neurons, is induced by the neuralizing agent noggin.
    Sharpe CR
    The International journal of developmental biology 42.6 (1998 Sep): 757-62.

    A role for S1P signalling in axon guidance in the Xenopus visual system.
    Holt CE
    Development (Cambridge, England) 135.2 (2008 Jan): 333-42.

    XBF-2 is a transcriptional repressor that converts ectoderm into neural tissue.
    Harland RM
    Development (Cambridge, England) 125.24 (1998 Dec): 5019-31.

    Math5 encodes a murine basic helix-loop-helix transcription factor expressed during early stages of retinal neurogenesis.
    Glaser T
    Development (Cambridge, England) 125.23 (1998 Dec): 4821-33.

    Growth cone interactions with a glial cell line from embryonic Xenopus retina.
    Harris WA
    Developmental biology 134.1 (1989 Jul): 158-74.

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