Zi 4G7
Catalog Fields
Clone ID/Product Name: Zi 4G7
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: ND
Ab Isotype: MIgG2a, kappa light chain
Gene Name:
Antibody Registry ID: AB_3094865
Clonality: Monoclonal
Immunogen: 72hr wasp-infected D. indianus hemocytes
Clone:
Immunogen Sequence: Full-length sequence
Myeloma Strain: Sp2/0
Epitope Mapped: No
Antigen Name: Zi Multinucleated Giant Hemocytes (MGH) and their filament derivatives}
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/22/2024
Antigen Molecular Weight: 37 kDa
Depositor: Ando, Istvan / Kurucz, Eva
Antigen Sequence:
Depositor Institution: HUN-REN Biological Research Centre, Szeged
Antigen Species: Zaprionus indianus
Depositor Notes: This antibody is specific for African Fig Fly multinucleated giant hemocytes (MGH) and their filament derivatives.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: African fig fly
Additional Information: The cells were fixed with acetone for 6 minutes, air dried and rehydrated with PBS solution for 10 minutes and blocked with PBS containing 0.1% BSA.
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Zi 4G7 was deposited to the DSHB by Ando, Istvan / Kurucz, Eva (DSHB Hybridoma Product Zi 4G7)
Storage and Handling Recommendations
Although many cell products are maintained at 4?C for years without loss of activity, shelf-life at 4?C is highly variable. For immediate use, short term storage at 4?C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ‘l for freezing at -20?C or -80?C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ‘g/ml. For western blots, the recommended concentration range is 0.2-0.5 ‘g/ml.
1 Reference
Initial Publication
Cellular Immune Response Involving Multinucleated Giant Hemocytes with Two-Step Genome Amplification in the Drosophilid Zaprionus indianus.
Andó I
Journal of innate immunity 12.3 (2020): 257-272.