anti-Bicaudal-D 4C2

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$40.00
SKU: anti-Bicaudal-D 4C2
View product citations for antibody anti-Bicaudal-D 4C2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Bicaudal-D
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Steward, R.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Waksman Institute, Rutgers University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Fail to detect BicD in some loss-of-function bicD alleles.
Antigen Molecular Weight: Predicted: 89kDa; Apparent: 97kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: BicD
Immunogen: Recombinant protein
Alternate Gene Names: almond; anon-EST; Bic-D; Dmel\CG6605
Alternate Antibody Name: 4C2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: N-terminal (aa 1-543)
Uniprot ID: P16568 
Immunogen Sequence: Full length protein
Entrez Gene ID: 35051 
Additional Characterization:  
Antibody Registry ID: AB_528103 
Additional Information: 4C2 and 1B11 (anti-bicaudal-D antibodies) bind to different epitopes on Bicaudal-D; 4C2 binds to a N-terminal epitope (aa 1-543) and 1B11 binds to a C-terminal epitope (aa 544-782). 4C2 and 1B11 have sometimes been combined to increase efficiency of immunoprecipitation.
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Bicaudal-D 4C2 was deposited to the DSHB by Steward, R. (DSHB Hybridoma Product anti-Bicaudal-D 4C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Requirement for phosphorylation and localization of the Bicaudal-D protein in Drosophila oocyte differentiation.
    Steward R
    Cell 67.5 (1991 Nov 29): 917-26.

    Systematic functional analysis of Bicaudal-D serine phosphorylation and intragenic suppression of a female sterile allele of BicD.
    Suter B
    PloS one 4.2 (2009): e4552.

    Functional domains of the Drosophila bicaudal-D protein.
    Steward R
    Genetics 154.2 (2000 Feb): 713-24.

    Rab6 mediates membrane organization and determinant localization during Drosophila oogenesis.
    Ephrussi A
    Development (Cambridge, England) 134.7 (2007 Apr): 1419-30.

    Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D.
    Suter B
    Development (Cambridge, England) 141.9 (2014 May): 1915-26.

    Interaction between Polo and BicD proteins links oocyte determination and meiosis control in Drosophila.
    Couderc JL
    Development (Cambridge, England) 133.20 (2006 Oct): 4005-13.

    BicaudalD actively regulates microtubule motor activity in lipid droplet transport.
    Gross SP
    PloS one 3.11 (2008): e3763.

    Pabp binds to the osk 3'UTR and specifically contributes to osk mRNA stability and oocyte accumulation.
    Suter B
    Developmental biology 357.2 (2011 Sep 15): 404-18.

    Rab6 and the secretory pathway affect oocyte polarity in Drosophila.
    Guichet A
    Development (Cambridge, England) 134.19 (2007 Oct): 3419-25.

    The golgin Lava lamp mediates dynein-based Golgi movements during Drosophila cellularization.
    Sisson JC
    Nature cell biology 7.6 (2005 Jun): 612-8.

    The sex determination master switch, Sex-lethal, responds to Hedgehog signaling in the Drosophila germline.
    Horabin JI
    Development (Cambridge, England) 128.14 (2001 Jul): 2649-60.

    Expression of the Sex-lethal gene is controlled at multiple levels during Drosophila oogenesis.
    Schedl P
    Development (Cambridge, England) 118.3 (1993 Jul): 797-812.

    IP References
    FB References
    Epitope Map References
    All References

    Systematic functional analysis of Bicaudal-D serine phosphorylation and intragenic suppression of a female sterile allele of BicD.
    Suter B
    PloS one 4.2 (2009): e4552.

    Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D.
    Suter B
    Development (Cambridge, England) 141.9 (2014 May): 1915-26.

    Interaction between Polo and BicD proteins links oocyte determination and meiosis control in Drosophila.
    Couderc JL
    Development (Cambridge, England) 133.20 (2006 Oct): 4005-13.

    BicaudalD actively regulates microtubule motor activity in lipid droplet transport.
    Gross SP
    PloS one 3.11 (2008): e3763.

    Pabp binds to the osk 3'UTR and specifically contributes to osk mRNA stability and oocyte accumulation.
    Suter B
    Developmental biology 357.2 (2011 Sep 15): 404-18.

    The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and establishment of polarity.
    Schedl P
    Genes & development 8.5 (1994 Mar 1): 598-613.

    The shut-down gene of Drosophila melanogaster encodes a novel FK506-binding protein essential for the formation of germline cysts during oogenesis.
    Steward R
    Genetics 156.1 (2000 Sep): 245-56.

    Global analysis of mRNA localization reveals a prominent role in organizing cellular architecture and function.
    Krause HM
    Cell 131.1 (2007 Oct 5): 174-87.

    A translation-independent role of oskar RNA in early Drosophila oogenesis.
    Ephrussi A
    Development (Cambridge, England) 133.15 (2006 Aug): 2827-33.

    Requirement for phosphorylation and localization of the Bicaudal-D protein in Drosophila oocyte differentiation.
    Steward R
    Cell 67.5 (1991 Nov 29): 917-26.

    Functional domains of the Drosophila bicaudal-D protein.
    Steward R
    Genetics 154.2 (2000 Feb): 713-24.

    Rab6 mediates membrane organization and determinant localization during Drosophila oogenesis.
    Ephrussi A
    Development (Cambridge, England) 134.7 (2007 Apr): 1419-30.

    Rab6 and the secretory pathway affect oocyte polarity in Drosophila.
    Guichet A
    Development (Cambridge, England) 134.19 (2007 Oct): 3419-25.

    The golgin Lava lamp mediates dynein-based Golgi movements during Drosophila cellularization.
    Sisson JC
    Nature cell biology 7.6 (2005 Jun): 612-8.

    The sex determination master switch, Sex-lethal, responds to Hedgehog signaling in the Drosophila germline.
    Horabin JI
    Development (Cambridge, England) 128.14 (2001 Jul): 2649-60.

    Expression of the Sex-lethal gene is controlled at multiple levels during Drosophila oogenesis.
    Schedl P
    Development (Cambridge, England) 118.3 (1993 Jul): 797-812.

    Conserved signals and machinery for RNA transport in Drosophila oogenesis and embryogenesis.
    Ish-Horowicz D
    Nature 414.6864 (2001 Dec 6): 611-6.

    Dynein anchors its mRNA cargo after apical transport in the Drosophila blastoderm embryo.
    Davis I
    Cell 122.1 (2005 Jul 15): 97-106.

    Egalitarian is a selective RNA-binding protein linking mRNA localization signals to the dynein motor.
    Bullock SL
    Genes & development 23.13 (2009 Jul 1): 1546-58.

    Stall encodes an ADAMTS metalloprotease and interacts genetically with Delta in Drosophila ovarian follicle formation.
    Cronmiller C
    Genetics 183.3 (2009 Nov): 1027-40.

    Motility screen identifies Drosophila IGF-II mRNA-binding protein--zipcode-binding protein acting in oogenesis and synaptogenesis.
    Hays TS
    PLoS genetics 4.2 (2008 Feb): e36.

    Drosophila Squid/hnRNP helps Dynein switch from a gurken mRNA transport motor to an ultrastructural static anchor in sponge bodies.
    Rabouille C
    Developmental cell 13.4 (2007 Oct): 523-38.

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