anti-Fen1 clone 19
Catalog Fields
Clone ID/Product Name: anti-Fen1 clone 19
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: Fen1
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_3095403
Uniprot ID: S4WYI8
Clonality: Monoclonal
Immunogen: E. coli expressed purified fenestrin protein
Clone:
Immunogen Sequence: Full-length sequence
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Fenestrin
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/24/2024
Antigen Molecular Weight: 64 kDa (apparent)
Depositor: E.S. Cole
Antigen Sequence:
Depositor Institution: St. Olaf College
Antigen Species: Tetrahymena pyroformis
Depositor Notes: Fenestrin is involved in nuclear exchange junctions in conjugating cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Tetrahymena thermophila
Additional Information: Hybridoma clone 19 was developed under contract by Proteogenix.
Recommended Applications: ELISA, Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Fen1 clone 19 was deposited to the DSHB by E.S. Cole (DSHB Hybridoma Product anti-Fen1 clone 19)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
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