anti-GST
Catalog Fields
Clone ID/Product Name: anti-GST
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: Rabbit IgG
Gene Name:
Antibody Registry ID: AB_3722706
Clonality: Polyclonal
Immunogen:
Clone:
Immunogen Sequence:
Myeloma Strain:
Epitope Mapped: No
Antigen Name: GST
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/13/2026
Antigen Molecular Weight: 26 kDa
Depositor: Endow, S.
Antigen Sequence:
Depositor Institution: Duke University
Antigen Species: S. japonicum
Depositor Notes: After purification, Ab was adjusted to OD=0.5 and used to bind GST-Ncd or other GST-motor fusion proteins to the coverslip surface for motility assays or for Western blots (1:1000-1:3000 dilution)
Host Species: Rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity:
Additional Information:
Recommended Applications: Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-GST was deposited to the DSHB by Endow, S. (DSHB Hybridoma Product anti-GST)
Storage and Handling Recommendations
Although many cell products are maintained at 4?C for years without loss of activity, shelf-life at 4?C is highly variable. For immediate use, short term storage at 4?C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 µl for freezing at -20?C or -80?C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 µg/ml. For western blots, the recommended concentration range is 0.2-0.5 µg/ml.
1 Reference
Initial Publication
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
Johnson KS
Gene 67.1 (1988 Jul 15): 31-40.