anti-Glucagon [4B1g]
![anti-Glucagon [4B1g]](https://dshb.biology.uiowa.edu/scs/img/2023_Logomark_Circle-Histo.png?resizeid=3&resizeh=600&resizew=600)
Catalog Fields
Clone ID/Product Name: anti-Glucagon [4B1g]
Available to For-Profits: No
Alternate Antibody Name: MEAH1m-4B1g
Gene Symbol: GCG
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_3675421
Uniprot ID: P01275
Entrez Gene ID: 2641
Clonality: Monoclonal
Immunogen: Peptide
Clone:
Immunogen Sequence: AQDFVQWLMNT
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Glucagon And Oxyntomodulin
Epitope Location or Sequence: Amino acids 71 - 82
Alternate Antigen Name:
Deposit Date: 12/2/2024
Antigen Molecular Weight: 12.8 kDa
Depositor: Hoofnagle, A.
Antigen Sequence:
Depositor Institution: University of Washington
Antigen Species: Human
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity:
Additional Information: Monoclonal antibodies to glucagon and oxyntomodulin were developed in collaboration with the Fred Hutchinson Antibody Technology Core. Mice were immunized to peptides HSQGTFTSDYSK and AQDFVQWLMNT. Both GCG and OXM hormones are derived from the same precursor protein, proglucagon. Oxyntomodulin contains the entire sequence of glucagon with an additional C-terminal extension. This mAb was generated against sequences present in both GCG and OXM and the antibody recognizes both hormones.
Predicted Species Reactivity:
Human Protein Atlas: https://www.proteinatlas.org/ENSG00000115263-GCG
Additional Characterization: https://docs.google.com/document/d/10HKuGkU3nZInAGoCgXu4uQnGLD-fLGAV/edit?usp=sharing&ouid=116960701580264900653&rtpof=true&sd=true
Recommended Applications: Immuno-MRM, Immuno-MS, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Glucagon [4B1g] was deposited to the DSHB by Hoofnagle, A. (DSHB Hybridoma Product anti-Glucagon [4B1g])
Storage and Handling Recommendations
Although many cell products are maintained at 4?C for years without loss of activity, shelf-life at 4?C is highly variable. For immediate use, short term storage at 4?C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 µl for freezing at -20?C or -80?C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 µg/ml. For western blots, the recommended concentration range is 0.2-0.5 µg/ml.
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