anti-dFmr1, 5B6

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$40.00
SKU: anti-dFmr1, 5B6
View product citations for antibody anti-dFmr1, 5B6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: dFmr1 protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Broadie, K.S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Vanderbilt University
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Multiple isforms; Predicted: between 60 and 76 kDa; Apparent: 65 to 80 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Fmr1
Immunogen: Recombinant GST-dFmr1 protein containing the N-terminal 600 aa
Alternate Gene Names: CG6203, dFMRP, dFXR1, dFXRP
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: RGG region: aa 470-507 of Uniprot ID Q9NFU0-2
Uniprot ID: Q9NFU0 
Immunogen Sequence: Partial protein
Entrez Gene ID: 37528 
Additional Characterization:  
Antibody Registry ID: AB_528253 
Additional Information: Epitope sequence conservation suggests this antibody should recognize all isoforms of dFmr1 protein.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-dFmr1, 5B6 was deposited to the DSHB by Broadie, K.S. (DSHB Hybridoma Product anti-dFmr1, 5B6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    The Drosophila FMRP and LARK RNA-binding proteins function together to regulate eye development and circadian behavior.
    Nelson DL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.41 (2008 Oct 8): 10200-5.

    IF References
    WB References
    IP References

    The Drosophila FMRP and LARK RNA-binding proteins function together to regulate eye development and circadian behavior.
    Nelson DL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.41 (2008 Oct 8): 10200-5.

    Fragile X mental retardation protein regulates translation by binding directly to the ribosome.
    Joseph S
    Molecular cell 54.3 (2014 May 8): 407-417.

    Dscam expression levels determine presynaptic arbor sizes in Drosophila sensory neurons.
    Ye B
    Neuron 78.5 (2013 Jun 5): 827-38.

    Overexpression of Down syndrome cell adhesion molecule impairs precise synaptic targeting.
    Chen BE
    Nature neuroscience 16.6 (2013 Jun): 677-82.

    Epitope Map References
    All References

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