anti-dFmr1, 5B6

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SKU: anti-dFmr1, 5B6

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DSHB Data Sheet

Catalog Fields

Product Name/ID: anti-dFmr1, 5B6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Fmr1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528253 
Uniprot ID: Q9NFU0 
RRID:  
Entrez Gene ID: 37528 
Clonality: Monoclonal
Immunogen: Recombinant GST-dFmr1 protein containing the N-terminal 600 aa
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: dFmr1 protein
Epitope Location or Sequence: RGG region: aa 470-507 of Uniprot ID Q9NFU0-2
Alternate Antigen Name:
Deposit Date: 10/7/2004
Antigen Molecular Weight: Multiple isforms; Predicted: between 60 and 76 kDa; Apparent: 65 to 80 kDa
Depositor: Broadie, K.S.
Antigen Sequence:
Depositor Institution: Vanderbilt University
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Epitope sequence conservation suggests this antibody should recognize all isoforms of dFmr1 protein.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-dFmr1, 5B6 was deposited to the DSHB by Broadie, K.S. (DSHB Hybridoma Product anti-dFmr1, 5B6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    The Drosophila FMRP and LARK RNA-binding proteins function together to regulate eye development and circadian behavior.
    Nelson DL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.41 (2008 Oct 8): 10200-5.

    IF References
    WB References
    IP References

    The Drosophila FMRP and LARK RNA-binding proteins function together to regulate eye development and circadian behavior.
    Nelson DL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.41 (2008 Oct 8): 10200-5.

    Fragile X mental retardation protein regulates translation by binding directly to the ribosome.
    Joseph S
    Molecular cell 54.3 (2014 May 8): 407-417.

    Dscam expression levels determine presynaptic arbor sizes in Drosophila sensory neurons.
    Ye B
    Neuron 78.5 (2013 Jun 5): 827-38.

    Overexpression of Down syndrome cell adhesion molecule impairs precise synaptic targeting.
    Chen BE
    Nature neuroscience 16.6 (2013 Jun): 677-82.

    Epitope Map References
    All References

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