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SKU: b4B4
View product citations for antibody b4B4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ATPase, (Na(+) K(+)) beta-1 subunit (avian)
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Avian
Depositors Notes: This antibody appears to recognize a linear epitope of the beta1-subunit of Na, K-ATPase exposed on the cell surface. Immunohistochemistry: works well on cryosections. Cros-reactivity with mammalian is unlikely.
Antigen Molecular Weight: Predicted: 35 kDa; Apparent: broad band at 45-60 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ATP1B1
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase beta-subunit.
Alternate Gene Names:
Alternate Antibody Name: beta4B4; mAb 4B4
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0 Ag8
Epitope Location or Sequence:
Uniprot ID: P08251 
Immunogen Sequence: Total protein
Entrez Gene ID: 396529 
Additional Characterization:  
Antibody Registry ID: AB_528095 
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
b4B4 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product b4B4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • WB References
  • All References
  • Initial Publication
    WB References

    Evidence for the formation of multimeric forms of the 5A11/HT7 antigen.
    Linser PJ
    Biochemical and biophysical research communications 229.1 (1996 Dec 4): 280-6.

    All References

    Evidence for the formation of multimeric forms of the 5A11/HT7 antigen.
    Linser PJ
    Biochemical and biophysical research communications 229.1 (1996 Dec 4): 280-6.

    Regulation of the (Na+ + K+)-ATPase in cultured chick skeletal muscle. Modulation of expression by the demand for ion transport.
    Fambrough DM
    The Journal of biological chemistry 261.21 (1986 Jul 25): 9990-9.

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