chi 1J

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SKU: chi 1J-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: chi 1J
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: chic
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528439 
Uniprot ID: P25843 
RRID:  
Entrez Gene ID: 33834 
Clonality: Monoclonal
Immunogen: Recombinant profilin fusion protein
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: SP2/2
Epitope Mapped: No
Antigen Name: Profilin/chickadee
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/12/2001
Antigen Molecular Weight: 13.7 kDa
Depositor: Cooley, L.
Antigen Sequence:
Depositor Institution: Yale University
Antigen Species: Drosophila
Depositor Notes: Drosophila profilin is also known as protein chickadee.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Drosophila
Additional Information: RRID:AB_528439 FBgn0000308
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
chi 1J was deposited to the DSHB by Cooley, L. (DSHB Hybridoma Product chi 1J)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

18 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Profilin mutations disrupt multiple actin-dependent processes during Drosophila development.
    Cooley L
    Development (Cambridge, England) 120.4 (1994 Apr): 717-28.

    IF References

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    A somatic permeability barrier around the germline is essential for Drosophila spermatogenesis.
    Tanentzapf G
    Development (Cambridge, England) 142.2 (2015 Jan 15): 268-81.

    The actin-binding protein profilin is required for germline stem cell maintenance and germ cell enclosure by somatic cyst cells.
    Fuller MT
    Development (Cambridge, England) 141.1 (2014 Jan): 73-82.

    Transcriptional regulation of Profilin during wound closure in Drosophila larvae.
    Galko MJ
    Journal of cell science 125.Pt 23 (2012 Dec 1): 5667-76.

    Drosophila sosie functions with β(H)-Spectrin and actin organizers in cell migration, epithelial morphogenesis and cortical stability.
    Suter B
    Biology open 1.10 (2012 Oct 15): 994-1005.

    AIP1 acts with cofilin to control actin dynamics during epithelial morphogenesis.
    Chen J
    Development (Cambridge, England) 139.19 (2012 Oct): 3561-71.

    Dissecting regulatory networks of filopodia formation in a Drosophila growth cone model.
    Prokop A
    PloS one 6.3 (2011 Mar 28): e18340.

    Expression of expanded polyglutamine targets profilin for degradation and alters actin dynamics.
    Di Prospero NA
    Neurobiology of disease 30.3 (2008 Jun): 365-374.

    Apical accumulation of the Drosophila PDGF/VEGF receptor ligands provides a mechanism for triggering localized actin polymerization.
    Shilo BZ
    Development (Cambridge, England) 131.9 (2004 May): 1939-48.

    Molecular requirements for actin-based lamella formation in Drosophila S2 cells.
    Vale RD
    The Journal of cell biology 162.6 (2003 Sep 15): 1079-88.

    WB References
    IHC References
    All References

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Developmental axon regrowth and primary neuron sprouting utilize distinct actin elongation factors.
    Schuldiner O
    The Journal of cell biology 219.5 (2020 May 4): .

    Dm Ime4 depletion affects permeability barrier and Chic function in Drosophila spermatogenesis.
    Hongay CF
    Mechanisms of development 164. (2020 Dec): 103650.

    A somatic permeability barrier around the germline is essential for Drosophila spermatogenesis.
    Tanentzapf G
    Development (Cambridge, England) 142.2 (2015 Jan 15): 268-81.

    The actin-binding protein profilin is required for germline stem cell maintenance and germ cell enclosure by somatic cyst cells.
    Fuller MT
    Development (Cambridge, England) 141.1 (2014 Jan): 73-82.

    Transcriptional regulation of Profilin during wound closure in Drosophila larvae.
    Galko MJ
    Journal of cell science 125.Pt 23 (2012 Dec 1): 5667-76.

    Drosophila sosie functions with β(H)-Spectrin and actin organizers in cell migration, epithelial morphogenesis and cortical stability.
    Suter B
    Biology open 1.10 (2012 Oct 15): 994-1005.

    AIP1 acts with cofilin to control actin dynamics during epithelial morphogenesis.
    Chen J
    Development (Cambridge, England) 139.19 (2012 Oct): 3561-71.

    Dissecting regulatory networks of filopodia formation in a Drosophila growth cone model.
    Prokop A
    PloS one 6.3 (2011 Mar 28): e18340.

    Expression of expanded polyglutamine targets profilin for degradation and alters actin dynamics.
    Di Prospero NA
    Neurobiology of disease 30.3 (2008 Jun): 365-374.

    Apical accumulation of the Drosophila PDGF/VEGF receptor ligands provides a mechanism for triggering localized actin polymerization.
    Shilo BZ
    Development (Cambridge, England) 131.9 (2004 May): 1939-48.

    Molecular requirements for actin-based lamella formation in Drosophila S2 cells.
    Vale RD
    The Journal of cell biology 162.6 (2003 Sep 15): 1079-88.

    Profilin mutations disrupt multiple actin-dependent processes during Drosophila development.
    Cooley L
    Development (Cambridge, England) 120.4 (1994 Apr): 717-28.

    [Morphologic and histochemical studies of the pineal gland in white rats during regeneration of the adrenals].
    Karasek M
    Endokrynologia Polska 19.5 (1968 Sep-Oct): 549-60.

    Drosophila BRUCE inhibits apoptosis through non-lysine ubiquitination of the IAP-antagonist REAPER.
    Ryoo HD
    Cell death and differentiation 19.3 (2012 Mar): 470-7.

    In vivo neuronal function of the fragile X mental retardation protein is regulated by phosphorylation.
    Broadie K
    Human molecular genetics 21.4 (2012 Feb 15): 900-15.

    Drosophila fragile X mental retardation protein developmentally regulates activity-dependent axon pruning.
    Broadie K
    Development (Cambridge, England) 135.8 (2008 Apr): 1547-57.

    Ythdf is a N6-methyladenosine reader that modulates Fmr1 target mRNA selection and restricts axonal growth in Drosophila.
    Roignant JY
    The EMBO journal 40.4 (2021 Feb 15): e104975.

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