lacunin 15D11

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SKU: lacunin 15D11
View product citations for antibody lacunin 15D11 on CiteAb

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Available: 10

DSHB Data Sheet

Catalog Fields

Antigen: lacunin
Available to For-Profits: Yes
Antigen Species: Manduca sexta
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Nardi, J.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Manduca sexta
Depositors Institution: University of Illinois
Antigen Molecular Weight: 360 kDa
Depositors Notes: Purification note: lacunin protein was purified on an immunoaffinity column, see Nardi et al. (1999).
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: developing adult moth wing epithelia
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Sp2/0
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID:  
Immunogen Sequence:
Antibody Registry ID: AB_10805534 
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
lacunin 15D11 was deposited to the DSHB by Nardi, J. (DSHB Hybridoma Product lacunin 15D11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FACS References
  • All References
  • Initial Publication
    IF References

    [Vena perforans varices].
    Nissl R
    Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin 103.3 (1965 Sep): 394-5.

    Clustering of adhesion receptors following exposure of insect blood cells to foreign surfaces.
    Kanost MR
    Journal of insect physiology 51.5 (2005 May): 555-64.

    WB References
    IHC References

    Hematopoietic organs of Manduca sexta and hemocyte lineages.
    Kanost MR
    Development genes and evolution 213.10 (2003 Oct): 477-91.

    Embryonic origins of the two main classes of hemocytes--granular cells and plasmatocytes--in Manduca sexta.
    Nardi JB
    Development genes and evolution 214.1 (2004 Jan): 19-28.

    FACS References

    [Vena perforans varices].
    Nissl R
    Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin 103.3 (1965 Sep): 394-5.

    All References

    Hematopoietic organs of Manduca sexta and hemocyte lineages.
    Kanost MR
    Development genes and evolution 213.10 (2003 Oct): 477-91.

    Embryonic origins of the two main classes of hemocytes--granular cells and plasmatocytes--in Manduca sexta.
    Nardi JB
    Development genes and evolution 214.1 (2004 Jan): 19-28.

    [Vena perforans varices].
    Nissl R
    Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin 103.3 (1965 Sep): 394-5.

    Clustering of adhesion receptors following exposure of insect blood cells to foreign surfaces.
    Kanost MR
    Journal of insect physiology 51.5 (2005 May): 555-64.

    Expression of lacunin, a large multidomain extracellular matrix protein, accompanies morphogenesis of epithelial monolayers in Manduca sexta.
    Robertson HM
    Insect biochemistry and molecular biology 29.10 (1999 Oct): 883-97.

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