lacunin 15D11

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SKU: lacunin 15D11
View product citations for antibody lacunin 15D11 on CiteAb

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Available: 11

DSHB Data Sheet

Catalog Fields

Antigen: lacunin
Hybridoma Cells Available: Yes
Antigen Species: Manduca sexta
Depositor: Nardi, J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Illinois
Positive Tested Species Reactivity: Manduca sexta
Depositors Notes: Purification note: lacunin protein was purified on an immunoaffinity column, see Nardi et al. (1999).
Antigen Molecular Weight: 360 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: developing adult moth wing epithelia
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_10805534 
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
lacunin 15D11 was deposited to the DSHB by Nardi, J. (DSHB Hybridoma Product lacunin 15D11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FACS References
  • All References
  • Initial Publication
    IF References

    [Vena perforans varices].
    Nissl R
    Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin 103.3 (1965 Sep): 394-5.

    Clustering of adhesion receptors following exposure of insect blood cells to foreign surfaces.
    Kanost MR
    Journal of insect physiology 51.5 (2005 May): 555-64.

    WB References
    IHC References

    Hematopoietic organs of Manduca sexta and hemocyte lineages.
    Kanost MR
    Development genes and evolution 213.10 (2003 Oct): 477-91.

    Embryonic origins of the two main classes of hemocytes--granular cells and plasmatocytes--in Manduca sexta.
    Nardi JB
    Development genes and evolution 214.1 (2004 Jan): 19-28.

    FACS References

    [Vena perforans varices].
    Nissl R
    Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin 103.3 (1965 Sep): 394-5.

    All References

    Hematopoietic organs of Manduca sexta and hemocyte lineages.
    Kanost MR
    Development genes and evolution 213.10 (2003 Oct): 477-91.

    Embryonic origins of the two main classes of hemocytes--granular cells and plasmatocytes--in Manduca sexta.
    Nardi JB
    Development genes and evolution 214.1 (2004 Jan): 19-28.

    [Vena perforans varices].
    Nissl R
    Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin 103.3 (1965 Sep): 394-5.

    Clustering of adhesion receptors following exposure of insect blood cells to foreign surfaces.
    Kanost MR
    Journal of insect physiology 51.5 (2005 May): 555-64.

    Expression of lacunin, a large multidomain extracellular matrix protein, accompanies morphogenesis of epithelial monolayers in Manduca sexta.
    Robertson HM
    Insect biochemistry and molecular biology 29.10 (1999 Oct): 883-97.

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