mAb 2F7

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$40.00
SKU: mAb 2F7
View product citations for antibody mAb 2F7 on CiteAb

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Available: 92

DSHB Data Sheet

Catalog Fields

Antigen: 2F7
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Kaprielian, Z.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Albert Einstein College of Medicine
Positive Tested Species Reactivity: Chick, Rat
Depositors Notes: Cell binding note: labels cultured neurons from dissociated E13.5 rat neural tubes. Immunoblotting note: labels several high-molecular weight (b/t 166 & 205 kDa) proteins, and to a lesser extent two proteins centered at about 75 kDa in a detergent-soluble membrane/cytoskeletal fraction derived from E14 rat spinal cord.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: cyclophosphamide immunosuppression method
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: HL1-653
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 2F7 was deposited to the DSHB by Kaprielian, Z. (DSHB Hybridoma Product mAb 2F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Characterization and distribution of a new cell surface marker of neuronal precursors.
    Kaprielian Z
    Developmental neuroscience 22.1-2 (2000): 154-66.

    All References

    Characterization and distribution of a new cell surface marker of neuronal precursors.
    Kaprielian Z
    Developmental neuroscience 22.1-2 (2000): 154-66.

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