mAb 2F7

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$45.00 to $425.00
SKU: mAb 2F7
View product citations for antibody mAb 2F7 on CiteAb

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Available: 15

DSHB Data Sheet

Catalog Fields

Antigen: 2F7
Available to For-Profits: Yes
Antigen Species: human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgM
Depositor: Kaprielian, Z.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Chick, Rat
Depositors Institution: Albert Einstein College of Medicine
Antigen Molecular Weight:
Depositors Notes: Cell binding note: labels cultured neurons from dissociated E13.5 rat neural tubes. Immunoblotting note: labels several high-molecular weight (b/t 166 & 205 kDa) proteins, and to a lesser extent two proteins centered at about 75 kDa in a detergent-soluble membrane/cytoskeletal fraction derived from E14 rat spinal cord.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: cyclophosphamide immunosuppression method
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: HL1-653
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID:  
Immunogen Sequence:
Antibody Registry ID: AB_1161647 
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 2F7 was deposited to the DSHB by Kaprielian, Z. (DSHB Hybridoma Product mAb 2F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Characterization and distribution of a new cell surface marker of neuronal precursors.
    Kaprielian Z
    Developmental neuroscience 22.1-2 (2000): 154-66.

    All References

    Characterization and distribution of a new cell surface marker of neuronal precursors.
    Kaprielian Z
    Developmental neuroscience 22.1-2 (2000): 154-66.

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