mAb 5B12.3
Catalog Fields
Antigen: F-actin capping protein alpha-1 & alpha-2 subunits
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Cooper, J.A.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Med. School, St. Louis
Positive Tested Species Reactivity: Chicken, Human, Mouse, Rat
Depositors Notes: For immunofluorescence staining, fixation with a glyoxal-based solution has been successful (PMID: 24319057/ 28899994) while paraformaldehyde has generally not succeeded. Glyoxal solutions can be prepared and used as described (PMID: 29146773) and are commercially available under the name GlyoFixx.
Predicted Species Reactivity:
Gene: CAPZA1
Immunogen: Recombinant GST-capping protein-alpha
Alternate Gene Names: CAPPA1, CAPZ, CAZ1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3x63 Ag8.653
Epitope Location or Sequence:
Uniprot ID: P13127
Immunogen Sequence: Full length
Entrez Gene ID: 829
Antibody Registry ID: AB_531816
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 5B12.3 was deposited to the DSHB by Cooper, J.A. (DSHB Hybridoma Product mAb 5B12.3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
10 References
Initial Publication
Dynamics of capping protein and actin assembly in vitro: uncapping barbed ends by polyphosphoinositides.
Cooper JA
The Journal of cell biology 135.1 (1996 Oct): 169-79.