mAb 5B12.3

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$40.00
SKU: mAb 5B12.3
View product citations for antibody mAb 5B12.3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: F-actin capping protein alpha-1 & alpha-2 subunits
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Cooper, J.A.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Med. School, St. Louis
Positive Tested Species Reactivity: Chicken, Human, Mouse, Rat
Depositors Notes: For immunofluorescence staining, fixation with a glyoxal-based solution has been successful (PMID: 24319057/ 28899994) while paraformaldehyde has generally not succeeded. Glyoxal solutions can be prepared and used as described (PMID: 29146773) and are commercially available under the name GlyoFixx.
Antigen Molecular Weight: 32.9 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CAPZA1
Immunogen: Recombinant GST-capping protein-alpha
Alternate Gene Names: CAPPA1, CAPZ, CAZ1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3x63 Ag8.653
Epitope Location or Sequence:
Uniprot ID: P13127 
Immunogen Sequence: Full length
Entrez Gene ID: 829 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 5B12.3 was deposited to the DSHB by Cooper, J.A. (DSHB Hybridoma Product mAb 5B12.3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • All References
  • Initial Publication
    IF References

    Actin capping protein is required for dendritic spine development and synapse formation.
    Zheng JQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.28 (2011 Jul 13): 10228-33.

    Correlation of actin crosslinker and capper expression levels with stereocilia growth phases.
    Barr-Gillespie PG
    Molecular & cellular proteomics : MCP 13.2 (2014 Feb): 606-20.

    WB References

    Dynamics of capping protein and actin assembly in vitro: uncapping barbed ends by polyphosphoinositides.
    Cooper JA
    The Journal of cell biology 135.1 (1996 Oct): 169-79.

    Rapid and efficient purification of actin from nonmuscle sources.
    Cooper JA
    Cell motility and the cytoskeleton 39.2 (1998): 166-71.

    Vertebrates have conserved capping protein alpha isoforms with specific expression patterns.
    Cooper JA
    Cell motility and the cytoskeleton 38.2 (1997): 120-32.

    Actin capping protein is required for dendritic spine development and synapse formation.
    Zheng JQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.28 (2011 Jul 13): 10228-33.

    The mouse formin, FRLalpha, slows actin filament barbed end elongation, competes with capping protein, accelerates polymerization from monomers, and severs filaments.
    Higgs HN
    The Journal of biological chemistry 279.19 (2004 May 7): 20076-87.

    The role of CKIP-1 in cell morphology depends on its interaction with actin-capping protein.
    Litchfield DW
    The Journal of biological chemistry 281.47 (2006 Nov 24): 36347-59.

    Actin-capping protein promotes microtubule stability by antagonizing the actin activity of mDia1.
    Gundersen GG
    Molecular biology of the cell 23.20 (2012 Oct): 4032-40.

    Correlation of actin crosslinker and capper expression levels with stereocilia growth phases.
    Barr-Gillespie PG
    Molecular & cellular proteomics : MCP 13.2 (2014 Feb): 606-20.

    IHC References

    Heterodimeric capping protein is required for stereocilia length and width regulation.
    Barr-Gillespie PG
    The Journal of cell biology 216.11 (2017 Nov 6): 3861-3881.

    IP References

    Heterodimeric capping protein is required for stereocilia length and width regulation.
    Barr-Gillespie PG
    The Journal of cell biology 216.11 (2017 Nov 6): 3861-3881.

    FB References

    Dynamics of capping protein and actin assembly in vitro: uncapping barbed ends by polyphosphoinositides.
    Cooper JA
    The Journal of cell biology 135.1 (1996 Oct): 169-79.

    Linking the T cell surface protein CD2 to the actin-capping protein CAPZ via CMS and CIN85.
    Brown MH
    The Journal of biological chemistry 278.25 (2003 Jun 20): 22396-403.

    All References

    Heterodimeric capping protein is required for stereocilia length and width regulation.
    Barr-Gillespie PG
    The Journal of cell biology 216.11 (2017 Nov 6): 3861-3881.

    Actin capping protein is required for dendritic spine development and synapse formation.
    Zheng JQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.28 (2011 Jul 13): 10228-33.

    Correlation of actin crosslinker and capper expression levels with stereocilia growth phases.
    Barr-Gillespie PG
    Molecular & cellular proteomics : MCP 13.2 (2014 Feb): 606-20.

    Dynamics of capping protein and actin assembly in vitro: uncapping barbed ends by polyphosphoinositides.
    Cooper JA
    The Journal of cell biology 135.1 (1996 Oct): 169-79.

    Rapid and efficient purification of actin from nonmuscle sources.
    Cooper JA
    Cell motility and the cytoskeleton 39.2 (1998): 166-71.

    Vertebrates have conserved capping protein alpha isoforms with specific expression patterns.
    Cooper JA
    Cell motility and the cytoskeleton 38.2 (1997): 120-32.

    The mouse formin, FRLalpha, slows actin filament barbed end elongation, competes with capping protein, accelerates polymerization from monomers, and severs filaments.
    Higgs HN
    The Journal of biological chemistry 279.19 (2004 May 7): 20076-87.

    The role of CKIP-1 in cell morphology depends on its interaction with actin-capping protein.
    Litchfield DW
    The Journal of biological chemistry 281.47 (2006 Nov 24): 36347-59.

    Actin-capping protein promotes microtubule stability by antagonizing the actin activity of mDia1.
    Gundersen GG
    Molecular biology of the cell 23.20 (2012 Oct): 4032-40.

    Linking the T cell surface protein CD2 to the actin-capping protein CAPZ via CMS and CIN85.
    Brown MH
    The Journal of biological chemistry 278.25 (2003 Jun 20): 22396-403.

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