mAb clone 2-4.3.25.11
Catalog Fields
Clone ID/Product Name: mAb clone 2-4.3.25.11
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: ACTG1
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_3712677
Uniprot ID: P63258
Entrez Gene ID: 404122
Clonality: Monoclonal
Immunogen:
Clone:
Immunogen Sequence:
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Purified bovine brain g-actin and KLH-conjugated peptide corresponding to amino-terminus of g-actin (Acetyl-EEEIAALVIDNGSGC-KLH)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/17/2025
Antigen Molecular Weight: 42 kDa
Depositor: Ervasti, J. M.
Antigen Sequence:
Depositor Institution: University of Wisconsin/Minnesota
Antigen Species: Bovine
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information:
Additional Characterization: https://www.pnas.org/doi/full/10.1073/pnas.0600980103
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb clone 2-4.3.25.11 was deposited to the DSHB by Ervasti, J. M. (DSHB Hybridoma Product mAb clone 2-4.3.25.11)
Storage and Handling Recommendations
Although many cell products are maintained at 4?C for years without loss of activity, shelf-life at 4?C is highly variable. For immediate use, short term storage at 4?C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 µl for freezing at -20?C or -80?C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 µg/ml. For western blots, the recommended concentration range is 0.2-0.5 µg/ml.
1 Reference
Initial Publication
Cytoplasmic gamma-actin contributes to a compensatory remodeling response in dystrophin-deficient muscle.
Ervasti JM
Proceedings of the National Academy of Sciences of the United States of America 103.14 (2006 Apr 4): 5385-90.