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SKU: rTSB D3-s
View product citations for antibody rTSB D3 on CiteAb

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Available: 29

DSHB Data Sheet

Catalog Fields

Antigen: Enolase superfamily member 1, Mitochondrial
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Dolnik, B.J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Roswell Park Cancer Institute
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes protein isoforms that are involved in the regulation of thymidylate synthase gene expression.
Antigen Molecular Weight: 49.7 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ENOSF1
Immunogen: Recombinant Protein
Alternate Gene Names: HSRTSBETA, rTS, TYMSAS
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: Q7L5Y1 
Immunogen Sequence: Full sequence
Entrez Gene ID: 55556 
Additional Characterization:  
Antibody Registry ID: AB_2617416 
Additional Information: This antibody detects four bands by western blot: 48 kDa (rTSbeta), 52kDa (rTSgamma), 42kDa (rTSalpha), and66kDa (summoylated rTS beta). RRID:AB_2617416
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
rTSB D3 was deposited to the DSHB by Dolnik, B.J. (DSHB Hybridoma Product rTSB D3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    ELISA References
    All References

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