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SKU: rTSB D3-s

In Stock

Available: 9

DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: rTSB D3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: ENOSF1
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2617416 
Uniprot ID: Q7L5Y1 
Entrez Gene ID: 55556 
Clonality: Monoclonal
Immunogen: Recombinant Protein
Immunogen Sequence: Full sequence
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Enolase superfamily member 1, Mitochondrial
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/9/2011
Antigen Molecular Weight: 49.7 kDa
Depositor: Dolnik, B.J.
Antigen Sequence:
Depositor Institution: Roswell Park Cancer Institute
Antigen Species: Human
Depositor Notes: This antibody recognizes protein isoforms that are involved in the regulation of thymidylate synthase gene expression.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: This antibody detects four bands by western blot: 48 kDa (rTSbeta), 52kDa (rTSgamma), 42kDa (rTSalpha), and66kDa (summoylated rTS beta). RRID:AB_2617416
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
rTSB D3 was deposited to the DSHB by Dolnik, B.J. (DSHB Hybridoma Product rTSB D3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    ELISA References
    All References

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