ro-62C2A8 anti-rough
Catalog Fields
Antigen: rough Drosophila protein
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Rubin, G.M.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: University of California, Berkeley
Antigen Molecular Weight: Predicted: 39 kDa
Depositors Notes: The protein rough is present in the nucleus of most or all of the cells in the morphogenetic furrow of the larval eye, and is limited to the R2, R3, R4, and R5 cells in the region posterior to the furrow. Antibody purified by ammonium salt precipitation may be more effective than supernatant. Use PEMP as a fixative.
Immunogen: Recmbinant fusion protein between protein A and aa 182-238 of Drosophila rough
Gene: ro
Alternate Antibody Name: mAbro1
Alternate Gene Names: CG6348
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: Yes
Uniprot ID: P10181
Epitope Location or Sequence: aa 182-238
Entrez Gene ID: 43234
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_528456
Recommended Applications: Immunofluorescence, Immunohistochemistry
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ro-62C2A8 anti-rough was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product ro-62C2A8 anti-rough)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
The homeo domain protein rough is expressed in a subset of cells in the developing Drosophila eye where it can specify photoreceptor cell subtype.
Rubin GM
Genes & development 4.5 (1990 May): 712-27.