ro-62C2A8 anti-rough
Catalog Fields
Antigen: rough Drosophila protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California at Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: The protein rough is present in the nucleus of most or all of the cells in the morphogenetic furrow of the larval eye, and is limited to the R2, R3, R4, and R5 cells in the region posterior to the furrow. Antibody purified by ammonium salt precipitation may be more effective than supernatant. Use PEMP as a fixative.
Predicted Species Reactivity:
Gene: ro
Immunogen: Recmbinant fusion protein between protein A and aa 182-238 of Drosophila rough
Alternate Gene Names: CG6348
Alternate Antibody Name: mAbro1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63Ag8.653
Epitope Location or Sequence: aa 182-238
Uniprot ID: P10181
Immunogen Sequence: Partial protein
Entrez Gene ID: 43234
Antibody Registry ID: AB_528456
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ro-62C2A8 anti-rough was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product ro-62C2A8 anti-rough)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
16 References
Initial Publication
The homeo domain protein rough is expressed in a subset of cells in the developing Drosophila eye where it can specify photoreceptor cell subtype.
Rubin GM
Genes & development 4.5 (1990 May): 712-27.