ro-62C2A8 anti-rough

(0) No Reviews yet
$40.00
SKU: ro-62C2A8 anti-rough
View product citations for antibody ro-62C2A8 anti-rough on CiteAb

In Stock

Available: 57

DSHB Data Sheet

Catalog Fields

Antigen: rough Drosophila protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Rubin, G.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California at Berkeley
Positive Tested Species Reactivity: Drosophila
Depositors Notes: The protein rough is present in the nucleus of most or all of the cells in the morphogenetic furrow of the larval eye, and is limited to the R2, R3, R4, and R5 cells in the region posterior to the furrow. Antibody purified by ammonium salt precipitation may be more effective than supernatant. Use PEMP as a fixative.
Antigen Molecular Weight: Predicted: 39 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ro
Immunogen: Recmbinant fusion protein between protein A and aa 182-238 of Drosophila rough
Alternate Gene Names: CG6348
Alternate Antibody Name: mAbro1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63Ag8.653
Epitope Location or Sequence: aa 182-238
Uniprot ID: P10181 
Immunogen Sequence: Partial protein
Entrez Gene ID: 43234 
Additional Characterization:  
Antibody Registry ID: AB_528456 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ro-62C2A8 anti-rough was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product ro-62C2A8 anti-rough)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Notchspl is deficient for inductive processes in the eye, and E(spl)D enhances split by interfering with proneural activity.
    Preiss A
    Developmental biology 208.2 (1999 Apr 15): 406-15.

    Proneural basic helix-loop-helix proteins and epidermal growth factor receptor signaling coordinately regulate cell type specification and cdk inhibitor expression during development.
    Du W
    Molecular and cellular biology 27.8 (2007 Apr): 2987-96.

    The novel plant homeodomain protein rhinoceros antagonizes Ras signaling in the Drosophila eye.
    Rebay I
    Genetics 165.4 (2003 Dec): 1993-2006.

    Drosophila EHBP1 regulates Scabrous secretion during Notch-mediated lateral inhibition.
    Bellen HJ
    Journal of cell science 126.Pt 16 (2013 Aug 15): 3686-96.

    Regulation of neurogenesis and epidermal growth factor receptor signaling by the insulin receptor/target of rapamycin pathway in Drosophila.
    Bateman JM
    Genetics 179.2 (2008 Jun): 843-53.

    Drosophila TRAP230/240 are essential coactivators for Atonal in retinal neurogenesis.
    Choi KW
    Developmental biology 308.2 (2007 Aug 15): 322-30.

    SOCS36E specifically interferes with Sevenless signaling during Drosophila eye development.
    Serras F
    Developmental biology 326.1 (2009 Feb 1): 212-23.

    Rap1 maintains adhesion between cells to affect Egfr signaling and planar cell polarity in Drosophila.
    Curtiss J
    Developmental biology 333.1 (2009 Sep 1): 143-60.

    Unkempt is negatively regulated by mTOR and uncouples neuronal differentiation from growth control.
    Bateman JM
    PLoS genetics 10.9 (2014 Sep): e1004624.

    Combined inactivation of pRB and hippo pathways induces dedifferentiation in the Drosophila retina.
    Frolov MV
    PLoS genetics 6.4 (2010 Apr 22): e1000918.

    Bar represses dPax2 and decapentaplegic to regulate cell fate and morphogenetic cell death in Drosophila eye.
    Choi KW
    PloS one 9.2 (2014): e88171.

    The atypical cadherin Flamingo links Frizzled and Notch signaling in planar polarity establishment in the Drosophila eye.
    Mlodzik M
    Developmental cell 2.5 (2002 May): 655-66.

    Control of photoreceptor axon target choice by transcriptional repression of Runt.
    Banerjee U
    Nature neuroscience 5.8 (2002 Aug): 746-50.

    Multiple functions of the EGF receptor in Drosophila eye development.
    Freeman M
    Current biology : CB 8.19 (1998 Sep 24): 1039-48.

    Conserved and divergent functions of Drosophila atonal, amphibian, and mammalian Ath5 genes.
    Brown NL
    Evolution & development 5.5 (2003 Sep-Oct): 532-41.

    IHC References
    Epitope Map References
    All References

    The homeo domain protein rough is expressed in a subset of cells in the developing Drosophila eye where it can specify photoreceptor cell subtype.
    Rubin GM
    Genes & development 4.5 (1990 May): 712-27.

    Notchspl is deficient for inductive processes in the eye, and E(spl)D enhances split by interfering with proneural activity.
    Preiss A
    Developmental biology 208.2 (1999 Apr 15): 406-15.

    Proneural basic helix-loop-helix proteins and epidermal growth factor receptor signaling coordinately regulate cell type specification and cdk inhibitor expression during development.
    Du W
    Molecular and cellular biology 27.8 (2007 Apr): 2987-96.

    The novel plant homeodomain protein rhinoceros antagonizes Ras signaling in the Drosophila eye.
    Rebay I
    Genetics 165.4 (2003 Dec): 1993-2006.

    Drosophila EHBP1 regulates Scabrous secretion during Notch-mediated lateral inhibition.
    Bellen HJ
    Journal of cell science 126.Pt 16 (2013 Aug 15): 3686-96.

    Regulation of neurogenesis and epidermal growth factor receptor signaling by the insulin receptor/target of rapamycin pathway in Drosophila.
    Bateman JM
    Genetics 179.2 (2008 Jun): 843-53.

    Drosophila TRAP230/240 are essential coactivators for Atonal in retinal neurogenesis.
    Choi KW
    Developmental biology 308.2 (2007 Aug 15): 322-30.

    SOCS36E specifically interferes with Sevenless signaling during Drosophila eye development.
    Serras F
    Developmental biology 326.1 (2009 Feb 1): 212-23.

    Rap1 maintains adhesion between cells to affect Egfr signaling and planar cell polarity in Drosophila.
    Curtiss J
    Developmental biology 333.1 (2009 Sep 1): 143-60.

    Unkempt is negatively regulated by mTOR and uncouples neuronal differentiation from growth control.
    Bateman JM
    PLoS genetics 10.9 (2014 Sep): e1004624.

    Combined inactivation of pRB and hippo pathways induces dedifferentiation in the Drosophila retina.
    Frolov MV
    PLoS genetics 6.4 (2010 Apr 22): e1000918.

    Bar represses dPax2 and decapentaplegic to regulate cell fate and morphogenetic cell death in Drosophila eye.
    Choi KW
    PloS one 9.2 (2014): e88171.

    The atypical cadherin Flamingo links Frizzled and Notch signaling in planar polarity establishment in the Drosophila eye.
    Mlodzik M
    Developmental cell 2.5 (2002 May): 655-66.

    Control of photoreceptor axon target choice by transcriptional repression of Runt.
    Banerjee U
    Nature neuroscience 5.8 (2002 Aug): 746-50.

    Multiple functions of the EGF receptor in Drosophila eye development.
    Freeman M
    Current biology : CB 8.19 (1998 Sep 24): 1039-48.

    Conserved and divergent functions of Drosophila atonal, amphibian, and mammalian Ath5 genes.
    Brown NL
    Evolution & development 5.5 (2003 Sep-Oct): 532-41.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review