UH3
Catalog Fields
Product Name/ID: UH3
Available to For-Profits: Yes
Alternate Antibody Name: CD107b
Gene Name: LAMP2
Ab Isotype: MIgG1
Gene Symbol:
Entrez Gene ID: 100689316
Clonality: Monoclonal
Immunogen: Membrane glycoproteins pre-extracted with saponin from Chinese hamster ovary (CHO) cells and triton-X100-solubilized. Glycoproteins were affinity-purified by binding to wheat-germ agglutinin. The fractions eluting with N-acetyl D-glucosamine were used.
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3U1
Epitope Mapped: No
Antigen Name: LAMP-2 (hamster)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/9/1997
Antigen Molecular Weight: Predicted: 42 kDa; Apparent: 110 kDa
Depositor: Granger, B.L. / Uthayakumar, S.
Antigen Sequence:
Depositor Institution: Montana State University
Antigen Species: Hamster
Depositor Notes: UH1 recognizes native, methanol-fixed, and PLP-fixed hamster antigen. Doesn't recognize rat, mouse or monkey antigens. UH3 Labels presumptive lysosomes (and late endosomes). For best immunochemistry results, fix cells with methanol or with (para)formaldehyde-lysine-periodate formulation; for the latter, permeabilize with saponin, not with Triton X-100 or NP-40. Unfixed cells that have been permeabilized with saponin can also be stained. For immunoprecipitation, the antigen solubilized with Triton X-100 will bind to GL2A7 coupled to a matrix.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Hamster
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
UH3 was deposited to the DSHB by Granger, B.L. / Uthayakumar, S. (DSHB Hybridoma Product UH3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
7 References
Initial Publication
Cell surface accumulation of overexpressed hamster lysosomal membrane glycoproteins.
Granger BL
Cellular & molecular biology research 41.5 (1995): 405-20.