Depositors Notes: Western blot was unsuccessful. For immunohistology see papers of developing and regenerating limbs and normal embryos. The antibody recognizes a cytoplasmic component of a population of blastemal cells that is lost when the cells differentiate into mature tissues. The growth of this population of cells is nerve dependent. (Intermediate filament protein or intermediate filament associated protein when staining fixed cultured blastemal cells.)
Additional Information: The immunization used 50-100 micrograms of protein for priming and for several boost for sacrificing. The origin of the antibody is notophthalmus viridescens. Works with Notopthalmus and Pleurodeles. Axolotl is weak. Triturus is weak. Nothing in higher vertebrates.
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
22/18 was deposited to the DSHB by Brockes, J.P. (DSHB Hybridoma Product 22/18)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.