6H1
Catalog Fields
Product Name/ID: 6H1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: MYH1
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_2314830
Uniprot ID: Q9TSE5
Entrez Gene ID: 100125991
Clonality: Monoclonal
Immunogen: Retractor bulbi (adult rabbit) crude muscle extract muscle co-absorbed with adjuvant peptide to gold particles as immunogen carrier.
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Myosin heavy chain, Type IIX
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/26/2007
Antigen Molecular Weight: ~200 kDa
Depositor: Lucas, C.
Antigen Sequence:
Depositor Institution: University of Sydney
Antigen Species: Rabbit
Depositor Notes: This is a very fragile IgM antibody. For optimum activity, we recommend these antibody products be kept at -20C or -80C either frozen in aliquots or with the addition of an equal volume of a cryoprotectant such as glycerol.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Feline, Guinea Pig, Human, Mouse, Rabbit, Rat
Additional Information: 6H1 has been successfully used to stain skeletal muscle paraffin sections. Low pH antigen retrieval and pre-treatment with Proteinase K is required. See PUBMED ID: 24924099.
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6H1 was deposited to the DSHB by Lucas, C. (DSHB Hybridoma Product 6H1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
34 References
Initial Publication
Monospecific antibodies against the three mammalian fast limb myosin heavy chains.
Hoh JF
Biochemical and biophysical research communications 272.1 (2000 May 27): 303-8.