Antigen: A6 Antigen in Biliary Epithelial & Oval cells
Hybridoma Cells Available: No
Antigen Species: Mouse
Depositor: Factor, V.M.
Host Species: rat
Depositors Institution: NIH
Positive Tested Species Reactivity: Mouse
Depositors Notes: This antibody is applicable on cultured cells, however the epitope is lost on primary cells after enzymatic digestion. In addition to biliary epithelium, A6 recognizes a wide variety of stratified and simple epithelium of ectodermal, mesodermal and endodermal origin in adult and embryonic tissues plus primary heptocarcinoma. See Table 3 in PMID 2292360.
Additional Information: This antibody recognizes the A6 antigen in frozen sections in unfixed, acetone, ethanol, methanol and paraformaldehyde fixed sections. Also, with antigen retrieval or enzymatic digestion with Proteinase K (20ug/ml in PBS;10-30 min), formalin-fixed paraffin embedded sections are sucessful labeled. RRID:AB_2618041
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
A6 BCM was deposited to the DSHB by Factor, V.M. (DSHB Hybridoma Product A6 BCM)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.