JONES
Catalog Fields
Product Name/ID: JONES
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_531870
Clonality: Monoclonal
Immunogen: Retinal epithelia homogenate (lightly fixed: 2% paraformaldehyde for 30 min) of fetal rats (E13-E20)
Clone:
Immunogen Sequence: GD3
Myeloma Strain: P3-NS1/1-Ag4-1
Epitope Mapped: Yes
Antigen Name: neural retinal gangliosides (9-0-acetyl-GD3)
Epitope Location or Sequence: 9-0-acetyl-GD3
Alternate Antigen Name:
Deposit Date: 3/29/1990
Antigen Molecular Weight:
Depositor: Constantine-Paton, M.
Antigen Sequence:
Depositor Institution: Massachusetts Institute of Technology
Antigen Species: Rat
Depositor Notes: JONES doesn't react with chicken tissue. It recognizes 9-0-acetyl-GD3 and a base-labile derivative of GQ1C, present in chloroform/methanol extracts from perinatal retina; neuraminidase sensitive. By immunohistochemistry, JONES binds to aldehyde-fixed sections of late fetal and neonatal rat retina.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Cat, Goat, Human, Monkey, Mouse, Opossum, Rat
Additional Information: Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JONES was deposited to the DSHB by Constantine-Paton, M. (DSHB Hybridoma Product JONES)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
A cell surface molecule distributed in a dorsoventral gradient in the perinatal rat retina.
Barnstable CJ
Nature 324.6096 (1986 Dec 4-10): 459-62.