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PCRP-BRWD1-1E12
$40.00


View product citations for antibody PCRP-BRWD1-1E12 on CiteAb
 
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Antigen: Bromodomain and WD repeat domain containing 1

Hybridoma Cells Available: Yes
Antigen Species: Human Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG2a Antigen Sequence:
Host Species: mouse Depositors Institution: National Institutes of Health
Positive Tested Species Reactivity: Human Depositors Notes: This antibody recognizes a member of theWD repeat protein family.
Antigen Molecular Weight: 262.9 kDa Human Protein Atlas: Link
Predicted Species Reactivity:   Gene: BRWD1
Immunogen: Recombinant peptide Alternate Gene Names: N143, WDR9
Alternate Antibody Name: Clonality: Monoclonal
Alternate Antigen Name: Epitope Mapped: No
Myeloma Strain: SP2/0-Ag14 Epitope Location or Sequence:
Uniprot ID: Immunogen Sequence: a.a. 1293-1450
Entrez Gene ID: Additional Characterization: Link
Antibody Registry ID: AB_2618456 Additional Information: RRID:AB_2618456  
Recommended Applications: Immunoprecipitation, Microarray
   
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-BRWD1-1E12 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-BRWD1-1E12)

Storage and Handling Recommendations Usage Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C. While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.  

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ChIP References
  • ELISA References
  • FFPE References
  • FB References
  • FACS References
  • Microarray References
  • Epitope Map References
  • Gel Supershift References
  • All References

Initial Publication

IHC References

IP References

IF References

WB References

ChIP References

ELISA References

FFPE References

FB References

FACS References

Microarray References

Epitope Map References

Gel Supershift References

All References

Developmental Studies Hybridoma Bank
University of Iowa, Department of Biology
028 Biology Building East
Iowa City, Iowa 52242-1324


Phone: 319-335-3826
Secure Fax: 319-359-4079
Email: dshb@uiowa.edu


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