Depositors Notes: Antigen retrieval (1% SDS for 5 min) required for immunostaining frozen sections. T9 is better than T4 in recognizing NKCC2, particularly by IF (PMID: 8572179, 12732642). T9 recpgmozes the 196kDa cotransporter exclusively on western blots [PMID: 8910547].
Gene: SLC12A1, SLC12A2
Immunogen: Recombinant 38kDa peptide fragment of carboxy terminus encoded by cDNA from T84 (human) epithelial Na-K-Cl cotransport protein
Alternate Gene Names: NKCC1 and NKCC2
Immunogen Sequence: human NKCC1 C-terminus (Met902-Ser1212)
Alternate Antibody Name:
Alternate Antigen Name: NKCC, Solute carrier family 12 members 1 and 2
Recommended Applications: Immunofluorescence, Western Blot
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T9 was deposited to the DSHB by Lytle, C. / Forbush III, B. (DSHB Hybridoma Product T9)
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