Antibody Name: b29
Antigen:ATPase, (Na(+) K(+)) beta-1 subunit (avian)
Catalog Fields
Identity
Clone ID / Antibody Name: b29
Alternate Antibody Name: beta29; mAb 29
Antigen Name: ATPase, (Na(+) K(+)) beta-1 subunit (avian)
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: ATP1B1
Entrez Gene ID: 396529
Antigen Sequence: —
Antigen Species: Chicken
Antigen Mol. Weight: Predicted: 35 kDa; Apparent: broad band at 45-60 kDa
Uniprot ID: P08251
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1, kappa light chain
Myeloma Strain: SP2/0 Ag8
Antibody Registry / RRID: AB_528096
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: purified adult chicken kidney (Na+, K+)-ATPase beta-subunit.
Immunogen Sequence: Total protein
Epitope Mapped: No
Epitope Location / Seq: —
Clone: —
Additional Info: —
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Avian
Predicted Reactivity: —
Depositor
Depositor Name: D.M. Fambrough
Institution: Johns Hopkins School of Medicine
Deposit Date: December 1, 1998
Depositor Notes: The epitope appears to be linear. Does not bind native beta subunits on cells, but does bind to some C-terminally truncated forms. This antibody preferentially recognizes partially denatured beta subunits.. This antibody is excellent for Immunoblotting but does not work for Immunoprecipitation or Immunohistochemistry. Cros-reactivity with mammalian is unlikely. Weaker than mAb 24 for immunoprecipitation of native beta subunit, but better than mAb 24 for truncated subunit [PMID 7901216].
Recommended Applications
IF
IP
WB
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