Depositors Notes: This antibody does not cross react with Ror1. The antigen is also known as neurotrophic tyrosine kinase, receptor-related 2 and is involved in cartilage growth, growth plate development and hair follicle patterning.
Immunogen: Ror2-MBP fusion protein
Alternate Gene Names: Ntrkr2, mRor2
Immunogen Sequence: NT 2535-2835/ C-terminal 100 amino acids
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Ror2 was deposited to the DSHB by Nusse, R. (DSHB Hybridoma Product Ror2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored either at -20°C or -80°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.