Immunogen: recombinant fusion protein, trpE fused to two different, nonoverlapping fragments of Drosophila fasII cDNA: a 1227 bp XhoI-HindIII fragment encopassing Ig domains 2-5 and a 496 bp SalI-ClaI fragment including fibronectin domain 2.
Alternate Gene Names: FasII; CG3665
Immunogen Sequence: Partial protein
Isotype: MIgG1, kappa light chain
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63.Ag8.653
Epitope Location or Sequence: extracellular domain of all four isoforms
Additional Information: The initial publication and the immunogen information are only putative and may or may not be correct. The depositor didn't specify any initial publication and didn't indicate the immunogen used. The mAb described in [PMID 1913818] has the generic name: anti-fasciclin II Mab.
Positive Tested Species Reactivity: Drosophila
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
34B3 anti-Fasciclin II was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 34B3 anti-Fasciclin II)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.