Clone ID / Name: 3C11 (anti SYNORF1)
Anti - Synapsin
Immunofluorescent image of a Drosophila neuromuscular junction (NMJ) labeled with DSHB antibody 3C11 (anti SYNORF1) against Synapsin (green). PMID: 31180325, Fig. 3A.
Catalog Fields
Identity
Clone ID / Name: 3C11 (anti SYNORF1)
Alternate Antibody Name: —
Antigen Name: Synapsin
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: Syn
Entrez Gene ID: 41247
Antigen Sequence: —
Antigen Species: Drosophila
Antigen Mol. Weight: Apparent: multiple bands at 70, 74, 80, and 143kDa
Uniprot ID: Q24546
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: MIgG2b, kappa light chain
Myeloma Strain: P3X63Ag8.653
Antibody Registry / RRID: AB_528479
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: GST-Synapsin-GST fusion protein expressed in E. coli and purified by glutathione affinity.
Immunogen Sequence: SYNORF1 which corresponds to Synapsin-1 from Uniprot
Epitope Mapped: Yes
Epitope Location / Seq: Peptide sequence LFGGMEVCGL which lies in the conserved C domain.
Clone: —
Additional Info: RRID: AB_528479
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Cephalopod, Crustacean, Drosophila, Insect, Invertebrate, Leech, Mosquito, Moth, Planaria, Rat
Predicted Reactivity: —
Depositor
Depositor Name: E. Buchner
Institution: Universitaetsklinikim Wuerzburg, Inst. of Clinical Neurobiology
Deposit Date: June 10, 2005
Depositor Notes: Cross-reacts with many invertebrate species. Stains neuropil and synaptic active zones. 3C11 recognizes the synapsin-1 cleaved fragment. Immunostaining is improved when fixed with zinc-formaldehyde instead of formaldehyde which allows for greater penetration of the 3C11 mAb in whole-mount brains. See PMID: 18585788.
Recommended Applications
FFPE
IF
IHC
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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