5D3
Catalog Fields
Product Name/ID: 5D3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Cdh1
Ab Isotype: MIgG2b
Gene Symbol:
Entrez Gene ID: 100337618
Clonality: Monoclonal
Immunogen: Purified E-cadherin extracellular domain (100 kDa trypsin fragment ectodomain) from Xenopus A6 kidney cell line.
Clone:
Immunogen Sequence: partial protein
Myeloma Strain: sp-2/0
Epitope Mapped: Yes
Antigen Name: Cadherin, E-
Epitope Location or Sequence: Extracellular domain
Alternate Antigen Name:
Deposit Date: 5/7/1993
Antigen Molecular Weight:
Depositor: Gumbiner, B.M.
Antigen Sequence:
Depositor Institution: University of Virginia
Antigen Species: Xenopus
Depositor Notes: Blocks epithelial cell-cell adhesion and cell junction formation. Stains ectoderm, all epithelia and regions of cell-cell contact. E-cadherin-specific by immunoblotting and immunoprecipitation.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila, Xenopus
Additional Information: 5D3 binds both the - and + (Ca++) conformations of E-cadherin
Recommended Applications: FFPE, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5D3 was deposited to the DSHB by Gumbiner, B.M. (DSHB Hybridoma Product 5D3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm.
Gumbiner B
The Journal of cell biology 108.6 (1989 Jun): 2449-58.