Clone ID / Name: 5D3
Anti - Cadherin, E-
Catalog Fields
Identity
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: MIgG2b
Myeloma Strain: sp-2/0
Antibody Registry / RRID: AB_528116
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Purified E-cadherin extracellular domain (100 kDa trypsin fragment ectodomain) from Xenopus A6 kidney cell line.
Immunogen Sequence: partial protein
Epitope Mapped: Yes
Epitope Location / Seq: Extracellular domain
Clone: —
Additional Info: 5D3 binds both the - and + (Ca++) conformations of E-cadherin
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Drosophila, Xenopus
Predicted Reactivity: —
Depositor
Depositor Name: B.M. Gumbiner
Institution: University of Virginia
Deposit Date: May 7, 1993
Depositor Notes: Blocks epithelial cell-cell adhesion and cell junction formation. Stains ectoderm, all epithelia and regions of cell-cell contact. E-cadherin-specific by immunoblotting and immunoprecipitation.
Recommended Applications
FFPE
Function Blocking
IF
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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