Positive Tested Species Reactivity: altricial fish larva, Chicken, Ferret, Fish, Frog, Human, Mouse, Rat, Zebrafish
Depositors Notes: MN, DRG & other nuclei characterized extensively by immunocytochemistry; works in paraformaldehyde-fixed and paraffin-embedded tissue; does not work in methanol or acetone (see Sanders et al., 2003, and Georgia et al., 2006) TEST
Predicted Species Reactivity:
Gene: Isl1 & Isl2
Immunogen: Recombinant fusion protein containing aa 178-349 of Isl1 protein (C-terminus)
Additional Information: also detects Islet-2. The protein region used for immunization lacks the LIM motifs shared with other proteins.
Recommended Applications: Chromatin Immunoprecipitation, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
39.4D5 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 39.4D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.