Antibody Name: A4.74
Antigen:Myosin heavy chain (human fast fibers)
Catalog Fields
Identity
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1
Myeloma Strain: 653
Antibody Registry / RRID: AB_528383
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: partially purified myosin (pyrophosphate extracted) from human adult (22 year old) skeletal muscle
Immunogen Sequence: —
Epitope Mapped: No
Epitope Location / Seq: —
Clone: —
Additional Info: stains MyHCIIa [PMID 7687223]. This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Bovine, C. elegans, Horse, Human, Llama, Porcine, Quail, Rodent
Predicted Reactivity: —
Depositor
Depositor Name: H.M. Blau
Institution: Stanford University, Baxter Lab for Stem Cell Biology
Deposit Date: August 25, 1993
Depositor Notes: Fusion: 1983. Recognizes all fast fibers in human. In rat, high affinity for IIA fibers, low affinity for IIX/D fibers, and doesn't stain IIB.
Recommended Applications
ELISA
IF
IHC
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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