Epitope Location or Sequence: CH1 has been reported to bind to the peptide region encoded by exon 9a of TPM genes [PMID: 24958154]; this exon is well conserved in all vertebrates. But, the binding of CH1 has also been described to be limited to alpha9a [PMID: 22069507].
Additional Information: CH1 reacts strongly with striated muscle forms of tropomyosin, including cardiac alpha-tropomyosin and skeletal alpha- and beta-tropomyosins, but not with tropomyosin isoforms from gizzard (smooth muscle) or chicken embryo fibroblasts. CH1 interferes with the binding of tropomyosin to actin. CH1 recognizes sarcomeric Tm soforms alpha-fast, beta, and alpha-slow with poor affinity for alpha-slow.
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CH1 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CH1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.