CIIC1
Catalog Fields
Product Name/ID: CIIC1
Available to For-Profits: Yes
Alternate Antibody Name: CIICI, C1
Gene Name: COL2A1
Ab Isotype: MIgG2a, kappa light chain
Gene Symbol:
Entrez Gene ID: 395069
Clonality: Monoclonal
Immunogen: native chick type I1 collagen
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: X63.Ag8.653
Epitope Mapped: Yes
Antigen Name: collagen type II
Epitope Location or Sequence: The ARGLT epitope is located at aa 359-363 of the human collagen II triple helical region
Alternate Antigen Name:
Deposit Date: 8/31/1989
Antigen Molecular Weight: Predicted: 125 kDa
Depositor: Holmdahl, R. / Rubin, K.
Antigen Sequence:
Depositor Institution: University of Uppsala
Antigen Species: chicken
Depositor Notes: Stains hyaluronidase-treated fetal and adult mouse and human cartilage in vitro as well as mouse cartilage in vivo. This antibody recognizes native triple-helical type II collagen. It does not bind to denatured type II collagen (PMID: 25005029).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bovine, Chick, Fish, Human, Mouse, Ovine, Rabbit, Rat, Xenopus
Additional Information: The CIIC1 antibody induces sub clinical arthritis if injected at low (0.5 mg) doses and clinical arthritis in mice if injected at higher doses and in combination with other antibodies (see [PMID: 16125192]).
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CIIC1 was deposited to the DSHB by Holmdahl, R. / Rubin, K. (DSHB Hybridoma Product CIIC1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
29 References
Initial Publication
Characterization of the antibody response in mice with type II collagen-induced arthritis, using monoclonal anti-type II collagen antibodies.
Wigzell H
Arthritis and rheumatism 29.3 (1986 Mar): 400-10.