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CIIC1
$40.00


View product citations for antibody CIIC1 on CiteAb
 
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Antigen: collagen type II

Hybridoma Cells Available: Yes
Antigen Species: chicken Depositor: Holmdahl, R. / Rubin, K.
Isotype: MIgG2a, kappa light chain Antigen Sequence:
Host Species: mouse Depositors Institution: University of Uppsala
Positive Tested Species Reactivity: Bovine, Chick, Fish, Human, Mouse, Ovine, Rabbit, Rat, Xenopus Depositors Notes: Stains hyaluronidase-treated fetal and adult mouse and human cartilage in vitro as well as mouse cartilage in vivo. This antibody recognizes native triple-helical type II collagen. It does not bind to denatured type II collagen (PMID: 25005029).
Antigen Molecular Weight: Predicted: 125 kDa Human Protein Atlas: Link
Predicted Species Reactivity:   Gene: COL2A1
Immunogen: native chick type I1 collagen Alternate Gene Names:
Alternate Antibody Name: CIICI, C1 Clonality: Monoclonal
Alternate Antigen Name: Epitope Mapped: Yes
Myeloma Strain: X63.Ag8.653 Epitope Location or Sequence: The ARGLT epitope is located at aa 359-363 of the human collagen II triple helical region
Uniprot ID: Immunogen Sequence: Full length protein
Entrez Gene ID: Additional Characterization: Link
Antibody Registry ID: AB_528164 Additional Information: The CIIC1 antibody induces sub clinical arthritis if injected at low (0.5 mg) doses and clinical arthritis in mice if injected at higher doses and in combination with other antibodies (see [PMID: 16125192]).  
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
   
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CIIC1 was deposited to the DSHB by Holmdahl, R. / Rubin, K. (DSHB Hybridoma Product CIIC1)

Storage and Handling Recommendations Usage Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C. While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.  

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ChIP References
  • ELISA References
  • FFPE References
  • FB References
  • FACS References
  • Microarray References
  • Epitope Map References
  • Gel Supershift References
  • All References

Initial Publication

IHC References

IP References

IF References

WB References

ChIP References

ELISA References

FFPE References

FB References

FACS References

Microarray References

Epitope Map References

Gel Supershift References

All References

Developmental Studies Hybridoma Bank
University of Iowa, Department of Biology
028 Biology Building East
Iowa City, Iowa 52242-1324


Phone: 319-335-3826
Secure Fax: 319-359-4079
Email: dshb@uiowa.edu


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