Pax3

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Supernatant 1.0 ml Concentrate 0.1 ml Frozen Cells 1 vial Growing Cells 1 vial

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DSHB Data Sheet

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Product Name/ID: Pax3

Available to For-Profits: Yes

Alternate Antibody Name: clone C2

Gene Name: Pax3

Ab Isotype: MIgG2a

Gene Symbol:

Antibody Registry ID: AB_528426

Uniprot ID: O13081

RRID:

Entrez Gene ID:

Clonality: Monoclonal

Immunogen: Recombinant partial protein (C-terminal region, aa 298-481)

Clone:

Immunogen Sequence: aa 298-481

Myeloma Strain:

Epitope Mapped: Yes

Antigen Name: Pax3

Epitope Location or Sequence: C-terminal region, aa 298-481

Alternate Antigen Name:

Deposit Date: 12/24/2003

Antigen Molecular Weight: Predicted: 53 kDa; Apparent: 53 kDa

Depositor: Ordahl, C.P.

Antigen Sequence:

Depositor Institution: University of California, San Francisco

Antigen Species: quail

Depositor Notes:

Host Species: mouse

Hybridoma Cells Available (Non-Profit): Yes

Confirmed Species Reactivity: Amphibian, Chicken, Fish, Human, Mouse, Quail, Rat, Zebrafish

Additional Information: The sequence for Uniprot ID O13081 appears to be missing the first five amino acids

Predicted Species Reactivity:

Human Protein Atlas:

Additional Characterization:

Recommended Applications: Chromatin Immunoprecipitation, FFPE, Gel Supershift, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

All cell products contain the antimicrobial ProClin. Click here for additional information.

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

Pax3 was deposited to the DSHB by Ordahl, C.P. (DSHB Hybridoma Product Pax3)

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

63 References

Initial Publication

IF References

WB References

IHC References

IP References

ChIP References

FFPE References

Epitope Map References

Gel Supershift References

All References

Initial Publication

Competence, specification and induction of Pax-3 in the trigeminal placode.
Bronner-Fraser M
Development (Cambridge, England) 126.1 (1999 Jan): 147-56.

Precocious terminal differentiation of premigratory limb muscle precursor cells requires positive signalling.
Ordahl CP
Developmental dynamics : an official publication of the American Association of Anatomists 229.3 (2004 Mar): 591-9.

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Pax3

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