DSHB-GFP3-1F5
Catalog Fields
Product Name/ID: DSHB-GFP3-1F5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: GFP
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2617424
Uniprot ID: P42212
Entrez Gene ID: 7011691
Clonality: Monoclonal
Immunogen: Recombinant GFP expressed in E. coli
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: Sp2/0-Ag14
Epitope Mapped: No
Antigen Name: Green fluorescent protein (GFP)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/6/2016
Antigen Molecular Weight: 26.8 kDa
Depositor: DSHB
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Aequorea victoria
Depositor Notes: DSHB-GFP3-1F5 is currently being validated for other applications.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Aequorea victoria
Additional Information: This antibody recognizes both denatured and native GFP. RRID:AB_2617424
Recommended Applications: Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-GFP3-1F5 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-GFP3-1F5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
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