Antibody Name: PMY-2A4

Antigen:puromycin

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$50.00

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SKU: PMY-2A4

Product Form :

Supernatant 1.0 ml

~ 31 – 43 μg/mL

Frozen Cells 1 vial

N/A

Growing Cells 1 vial

N/A

iConcentration varies between lots. Lots are distributed in the order they are produced.

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Available: 1

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Catalog Fields

Identity

Clone ID / Antibody Name: PMY-2A4

Alternate Antibody Name: clone 4A12

Antigen Name: puromycin

Alternate Antigen Name: —

Gene Symbol: —

Gene Name: —

Entrez Gene ID: —

Antigen Sequence: —

Antigen Species: Streptomyces alboniger

Antigen Mol. Weight: 0.5kDa

Uniprot ID: —

Human Protein Atlas: —

Antibody Properties

Host Species: Mouse

Clonality: Monoclonal

Isotype: Mouse IgG2c

Myeloma Strain: SP2

Antibody Registry / RRID: AB_2619605

Available to For-Profits: Yes

Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.

Immunogen & Epitope

Immunogen: Puromycin (coupled to KLH) from Streptomyces alboniger bacterium. Chemical Composition: 3'-(alpha-Amino-p-methoxyhydrocinnamamido)-3'-deoxy-N,N-dimethyladenosine, 2HC1.

Immunogen Sequence: —

Epitope Mapped: Yes

Epitope Location / Seq: —

Clone: —

Additional Info: Puromycin is a structural analog of aminoacyl tRNAs that can be incorporated into nascent polypeptide chain and blocks elongation. This antibody is used to detect the subcellular localization of actively translating ribosomes by the ribopuromycylation method (RPM), and localizes puromycylated nascent chains on ribosomes immobilized by chain elongation inhibitors in living cells prior to processing for intracellular immunofluorescence and/or western blots. RRID:AB_2619605

Add. Characterization: —

Species Reactivity

Confirmed Reactivity: All

Predicted Reactivity: —

Depositor

Depositor Name: Jonathan Yewdell

Institution: National Institutes of Health

Deposit Date: March 9, 2012

Depositor Notes: Fusion date: 07/21/2010. Fixation, 4% paraformaldehyde. The antigen puromycin inhibits translation by covalently associating with nascent chain within the ribosome. 2A4 allows puromycin detection in this condition. 2A4 also detects any puromycylated peptide. Medium: RPMI 1640 / Glutamine / Na pyruvate / 10% fetal bovine serum / Penicillin-streptomycin /gentamycin

Recommended Applications

ELISA

FACS

Each lab must optimize concentration per application and species. Full usage recommendations →

UniProt DataUniProtNo UniProt ID

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No UniProt ID is available for this product.

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Antibody Name: PMY-2A4

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