PMY-2A4
Catalog Fields
Clone ID/Product Name: PMY-2A4
Available to For-Profits: Yes
Alternate Antibody Name: clone 4A12
Gene Symbol:
Ab Isotype: MIgG2c
Gene Name:
Antibody Registry ID: AB_2619605
Clonality: Monoclonal
Immunogen: Puromycin (coupled to KLH) from Streptomyces alboniger bacterium. Chemical Composition: 3'-(alpha-Amino-p-methoxyhydrocinnamamido)-3'-deoxy-N,N-dimethyladenosine, 2HC1.
Clone:
Immunogen Sequence:
Myeloma Strain: SP2
Epitope Mapped: Yes
Antigen Name: puromycin
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/9/2012
Antigen Molecular Weight: 0.5kDa
Depositor: Yewdell, Jonathan
Antigen Sequence:
Depositor Institution: National Institutes of Health
Antigen Species: Streptomyces alboniger
Depositor Notes: Fusion date: 07/21/2010. Fixation, 4% paraformaldehyde. The antigen puromycin inhibits translation by covalently associating with nascent chain within the ribosome. 2A4 allows puromycin detection in this condition. 2A4 also detects any puromycylated peptide. Medium: RPMI 1640 / Glutamine / Na pyruvate / 10% fetal bovine serum / Penicillin-streptomycin /gentamycin
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: All
Additional Information: Puromycin is a structural analog of aminoacyl tRNAs that can be incorporated into nascent polypeptide chain and blocks elongation. This antibody is used to detect the subcellular localization of actively translating ribosomes by the ribopuromycylation method (RPM), and localizes puromycylated nascent chains on ribosomes immobilized by chain elongation inhibitors in living cells prior to processing for intracellular immunofluorescence and/or western blots. RRID:AB_2619605
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunoprecipitation, Western Blot