Seven-up 6F7
Catalog Fields
Product Name/ID: Seven-up 6F7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: SVP
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2618081
Uniprot ID: P16376
Entrez Gene ID: 41491
Clonality: Monoclonal
Immunogen: Recombinant peptide
Clone:
Immunogen Sequence: N-terminal region including DNA binding domain
Myeloma Strain:
Epitope Mapped: Yes
Antigen Name: Seven-up
Epitope Location or Sequence: a.a. 150-170
Alternate Antigen Name:
Deposit Date: 10/8/2015
Antigen Molecular Weight: 76.8 kDa
Depositor: Hiromi, Y. / Hondo, T. / Kanda, H.
Antigen Sequence:
Depositor Institution: Keio University School of Medicine
Antigen Species: Drosophila
Depositor Notes: Seven-up is the highly conserved Drosophila homolog of the human TF-COUP2,a nuclear receptor. The Drosophila protein is over 90% identical to the human homolog.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: RRID:AB_2618081
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Seven-up 6F7 was deposited to the DSHB by Hiromi, Y. / Hondo, T. / Kanda, H. (DSHB Hybridoma Product Seven-up 6F7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
1 Reference