12C5

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SKU: 12C5-s

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Supernatant 1.0 ml

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DSHB Data Sheet

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Product Name/ID: 12C5

Available to For-Profits: Yes

Alternate Antibody Name:

Gene Name: VCAN

Ab Isotype: MIgG1

Gene Symbol:

Antibody Registry ID: AB_528503

Uniprot ID: P13611

RRID:

Entrez Gene ID: 1462

Clonality: Monoclonal

Immunogen: hyaluronate-binding region of versican (aka _GHAP_)

Clone:

Immunogen Sequence: same as antigen sequence.

Myeloma Strain: NS-1

Epitope Mapped: Yes

Antigen Name: versican (hyaluronate-binding region)

Epitope Location or Sequence: G1 domain

Alternate Antigen Name:

Deposit Date: 1/18/1995

Antigen Molecular Weight: Apparent: >250kD

Depositor: Asher, R.A.

Antigen Sequence: Conserved sequence in N-terminal G1 domain

Depositor Institution: Cambridge Centre for Brain Repair

Antigen Species: Human

Depositor Notes: Blot: yes, but sensitive to reduction

Host Species: mouse

Hybridoma Cells Available (Non-Profit): No

Confirmed Species Reactivity: Bovine, Canine, Human, Porcine, Rat

Additional Information:

Predicted Species Reactivity:

Human Protein Atlas:

Additional Characterization:

Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Microarray, Western Blot

All cell products contain the antimicrobial ProClin. Click here for additional information.

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

12C5 was deposited to the DSHB by Asher, R.A. (DSHB Hybridoma Product 12C5)

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

32 References

Initial Publication

IF References

WB References

IHC References

IP References

ELISA References

FFPE References

FB References

Microarray References

Epitope Map References

All References

Initial Publication

Extracellular matrix of central nervous system white matter: demonstration of an hyaluronate-protein complex.
Bignami A
Journal of neuroscience research 28.3 (1991 Mar): 410-21.

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12C5

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