AON-1
Catalog Fields
Product Name/ID: AON-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: SPARC
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Entrez Gene ID: 282077
Clonality: Monoclonal
Immunogen: Purified bovine osteonectin (SPARC)
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: x63-AG8.653
Epitope Mapped: No
Antigen Name: Osteonectin (SPARC)
Epitope Location or Sequence: The epitope bound by AON-1 is cryptic and often requires tissue pretreatment for exposure. See PMID: 19435834 & 2476206.
Alternate Antigen Name:
Deposit Date: 7/13/1990
Antigen Molecular Weight: 42 kDa
Depositor: Termine, J.D.
Antigen Sequence:
Depositor Institution: NIDR, NIH, Bone Research Branch
Antigen Species: Bovine
Depositor Notes: The isotype of this monoclonal antibody was originally reported as IgG3 (PMID: 2476206). This antibody recognizes osteonectin (SPARC)-containing cartilages in invertebrate species. See PMID: 16351944.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Acorn worm, Bovine, Canine, Human, Monkey, Pig, Rabbit, Rat
Additional Information: RRID:AB_528421
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AON-1 was deposited to the DSHB by Termine, J.D. (DSHB Hybridoma Product AON-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Monoclonal antibodies against osteonectin show conservation of epitopes across species.
Termine JD
Calcified tissue international 45.2 (1989 Aug): 74-80.