Positive Tested Species Reactivity: Drosophila melanogaster, Drosophila virilis
Depositors Notes: crossreacts with Drosophila virilis, not with mouse or C. elegans. Crumbs protein is expressed in all epithelia of ectodermal origin in the Drosophila embryo from gastrulation onwards and in all imaginal discs and the follicle epithelium. It is restricted to a region apical to the zonula adherens. Good apical marker for epithelial cells.
Predicted Species Reactivity:
Immunogen: Recombinant fusion protein between beta-galactosidase and aa 737-1703 of Drosophila Crumbs protein.
Additional Information: For experiments involving live cells and tissue, Cq4 supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or firstname.lastname@example.org for special ordering instructions.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Cq4 was deposited to the DSHB by Knust, E. (DSHB Hybridoma Product Cq4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.