Antigen: plasmalemma vesicle-associated protein (PLVAP)
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Butcher, E.C.
Host Species: rat
Depositors Institution: Stanford University School of Medicine
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: Binds to murine endothelial cells. most or all EC except for capillaries in skeletal and cardiac muscle, and vessels in the CNS. The antigen is downregulated during CNS development in association with formation of the blood brain barrier, and is induced in negative vessels in muscle and CNS in association with inflammation.
Additional Information: Marker of fenestrated vasculature
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MECA-32 was deposited to the DSHB by Butcher, E.C. (DSHB Hybridoma Product MECA-32)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.