Antibody Name: N2.261
Antigen:Myosin heavy chain (human all fibers except fast IIB)
Immunofluorescent image of a zebrafish heart at 7dpa labeled with DSHB antibody N2.261 against Myosin heavy chain, human, all fibers except fast IIB (red). PMID: 32877671, Fig. S4E.
Catalog Fields
Identity
Clone ID / Antibody Name: N2.261
Alternate Antibody Name: Anti-MyHCI/3rd+IIa
Antigen Name: Myosin heavy chain (human all fibers except fast IIB)
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: MYH7
Entrez Gene ID: 4625
Antigen Sequence: —
Antigen Species: Human
Antigen Mol. Weight: 220kDa
Uniprot ID: P12883
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1, kappa light chain
Myeloma Strain: SP2/2
Antibody Registry / RRID: AB_531790
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: partially purified myosin (pyrophosphate extracted) from human neonatal (5 days old) skeletal muscle
Immunogen Sequence: Full length protein
Epitope Mapped: Yes
Epitope Location / Seq: N-terminal first 100 aa
Clone: —
Additional Info: Stains MyHCIIa and third developmental stage MyHCI, and the epitope on slow MHC is different from that of mAbs A4.951 and A4.951 [PMID 7687223]. Stains all fibers except fast IIb [PMID 3342447; PMID 11005719]. This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive. RRID: AB_531790
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Human, Rodent, Zebrafish
Predicted Reactivity: —
Depositor
Depositor Name: H.M. Blau
Institution: Stanford University School of Medicine
Deposit Date: August 25, 1993
Depositor Notes: Fusion: 1983.
Recommended Applications
ELISA
IF
IHC
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
▼
Could not load UniProt data for P12883