Antigen: Myosin heavy chain, slow developmental (sd-MyHC)
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Stockdale, F.E.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: Stanford University School of Medicine
Positive Tested Species Reactivity: Chicken, Human, Mouse, Quail, Rat, Zebrafish
Depositors Notes: This antigen is also known as myosin heavy chain, cardiac muscle alpha isoform. S46 preferentially stains slow tonic myosin heavy chain in humans including extraocular, intrafusal and the atrium of the heart.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
S46 was deposited to the DSHB by Stockdale, F.E. (DSHB Hybridoma Product S46)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.