Depositors Notes: Reacted with all tested vertebrate species. The 40.2D6 monoclonal antibody is often combined with monoclonal antibodies 39.3F7, 39.4D5, and 40.3A4 to create a efficient cocktail for ChIP and/or gel shift [PMID 19502415].
Immunogen: C-terminal of rat Islet-1 (aa 178-349); recombinant .
Alternate Gene Names: Isl-1; isl-1=homeobox
Immunogen Sequence: Recombinant C-terminal of rat Islet-1 (aa 178-349)
Positive Tested Species Reactivity: altricial fish larva, Chicken, Fish, Frog, Human, Mouse, Newt, Planaria, Porcine, Rat, Reptile, Vertebrates, Xenopus, Zebrafish
Recommended Applications: Chromatin Immunoprecipitation, FACS, FFPE, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
40.2D6 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 40.2D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.