mAB sca1
Catalog Fields
Product Name/ID: mAB sca1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: sca
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Entrez Gene ID: 36411
Clonality: Monoclonal
Immunogen: Recombinant sca-GST fusion protein
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3X63-Ag8.653
Epitope Mapped: Yes
Antigen Name: scabrous protein, Drosophila
Epitope Location or Sequence: aa 41-275
Alternate Antigen Name:
Deposit Date: 8/30/1995
Antigen Molecular Weight: Predicted: 90 kDa; Apparent: 300 kDa
Depositor: Baker, N.
Antigen Sequence:
Depositor Institution: Albert Einstein College of Medicine
Antigen Species: Drosophila
Depositor Notes: Monospecific in immunohistology or immunoblotting
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAB sca1 was deposited to the DSHB by Baker, N. (DSHB Hybridoma Product mAB sca1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
14 References
Initial Publication
The scabrous gene encodes a secreted glycoprotein dimer and regulates proneural development in Drosophila eyes.
Baker NE
Molecular and cellular biology 16.3 (1996 Mar): 1179-88.