34C
Catalog Fields
Product Name/ID: 34C
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: RYR1/RYR2
Ab Isotype: MIgG1
Gene Symbol:
Entrez Gene ID: 6261
Clonality: Monoclonal
Immunogen: Microsomal membrane preparation from skeletal muscle
Clone:
Immunogen Sequence: Purified alpha and beta ryanodine receptor
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: Yes
Antigen Name: Ryanodine receptor
Epitope Location or Sequence: a.a. 2756-2803 (RyR1) and a.a. 2722-2769 (RyR2) )
Alternate Antigen Name:
Deposit Date: 2/29/1996
Antigen Molecular Weight: 562.7 kDa
Depositor: Airey, J. / Sutko, J.
Antigen Sequence:
Depositor Institution: University of Nevada
Antigen Species: chicken
Depositor Notes: This antigen is a calcium release channel localized in the sarcoplasmic reticulum.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Amphibian, Avian, Fish, Mammal
Additional Information: RRID:AB_528457
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
34C was deposited to the DSHB by Airey, J. / Sutko, J. (DSHB Hybridoma Product 34C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
Identification and localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle.
Sutko JL
The Journal of biological chemistry 265.24 (1990 Aug 25): 14187-94.